Evidence map›Paper›PMID 28515938›Full record

ArticleTherapeutic advances in vaccines2017

Accounting for adjuvant-induced artifacts in the characterization of vaccine formulations by polyacrylamide gel electrophoresis.

Virginie Jakob, Livia Brunner, Christophe Barnier-Quer, Molly Blust, Nicolas Collin, Lauren Carter, Darrick Carter, Kelly M Rausch, Christopher B Fox

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Therapeutic advances in vaccines, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Immunogenic characterization of AlPOClinical and experimental vaccine research · 2023
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Virginie JakobVaccine Formulation Laboratory, University of Lausanne, Epalinges, Switzerland.
Livia BrunnerVaccine Formulation Laboratory, University of Lausanne, Epalinges, Switzerland.
Christophe Barnier-QuerVaccine Formulation Laboratory, University of Lausanne, Epalinges, Switzerland.
Molly BlustInfectious Disease Research Institute, Seattle, WA, USA.
Nicolas CollinVaccine Formulation Laboratory, University of Lausanne, Epalinges, Switzerland.
Lauren CarterInfectious Disease Research Institute, Seattle, WA, USA.
Darrick CarterInfectious Disease Research Institute, Seattle, WA, USA, and Department of Global Health, University of Washington, Seattle, WA, USA.
Kelly M RauschLaboratory of Malaria Immunology and Vaccinology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA.
Christopher B FoxInfectious Disease Research Institute, Department of Global Health, University of Washington, 1616 Eastlake Ave E Ste 400, Seattle, WA 98102, USA.
University of Lausanne · CHInfectious Disease Research Institute · USUniversity of Washington · USNational Institute of Allergy and Infectious Diseases · US

Funding

NIAID NIH HHS HHSN272201400041C
6 · The paper itself

Abstract

objectivesSeveral vaccine adjuvants comprise complex nano- or micro-particle formulations, such as oil-in-water emulsions. In order to characterize interactions and compatibility of oil-in-water emulsion adjuvants with protein antigens in vaccines, effective protein characterization methods that can accommodate potential interference from high concentrations of lipid-based particles are needed.

methodsSodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a standard protein characterization technique which is affected by the presence of adjuvants such as oil-in-water emulsions. In this article, we investigate variations in SDS-PAGE methods that result in a reduction of adjuvant-induced staining artifacts. We have investigated whether the SDS method or the adjuvant composition were the reason for these artifacts and succeeded in reducing the artifacts with a modified sample preparation and different staining procedures.

resultsThe best results were obtained by using gold staining or silver staining instead of a Coomassie Blue staining procedure. Moreover, the replacement of the dilution buffer (20% SDS to disrupt emulsion) by alternative detergents such as Tween® 80 and Triton® X-100 removed adjuvant-induced streaking artifacts at the top of the gel.

conclusionsThese methods may be useful for improving characterization approaches of antigen-adjuvant mixtures by SDS-PAGE.

Indexed as

oil-in-water emulsionpolyacrylamide gel electrophoresisvaccine adjuvant characterizationvaccine adjuvant formulation

Identifiers

PMID28515938
PMCPMC5418890
OpenAlexW2610613016

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.