ArticleBiotechnology for biofuels2017
Improving
Article in Biotechnology for biofuels, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed, 77 citations in OpenAlex.
- Characterization of microbial community and yeast functional traits inFood chemistry: X · 2026Article
- Physiological characterization and safety assessment of thermotolerant Lodderomyces elongisporus in sauce-flavored Daqu: based on genomic insight.Antonie van Leeuwenhoek · 2026Article
- Engineering microorganisms for enhanced tolerance to toxic end-products and intermediates.FEMS microbiology reviews · 2025Review
- Tuning fungal promoters for the expression of eukaryotic proteins.World journal of microbiology & biotechnology · 2024Review
- Improving furfural tolerance in a xylose-fermenting yeast Spathaspora passalidarum CMUWF1-2 via adaptive laboratory evolution.Microbial cell factories · 2024Article
- Strategies to increase the robustness of microbial cell factories.Advanced biotechnology · 2024Review
- Engineered yeasts and lignocellulosic biomaterials: shaping a new dimension for biorefinery and global bioeconomy.Bioengineered · 2023Review
- A panoramic view of technological landscape for bioethanol production from various generations of feedstocks.Bioengineered · 2023Review
- High-temperature ethanol fermentation from pineapple waste hydrolysate and gene expression analysis of thermotolerant yeast Saccharomyces cerevisiae.Scientific reports · 2022Article
- Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass inBiomolecules · 2022Review
- Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels.Bioengineered · 2022Review
- Strategies to increase tolerance and robustness of industrial microorganisms.Synthetic and systems biotechnology · 2022Article
- The Association of Rpb4 with RNA Polymerase II Depends on CTD Ser5P Phosphatase Rtr1 and Influences mRNA Decay inInternational journal of molecular sciences · 2022Article
- BioprospectingFrontiers in bioengineering and biotechnology · 2022Review
- The Model SystemLife (Basel, Switzerland) · 2020Review
- Do Metabolomics and Taxonomic Barcode Markers Tell the Same Story about the Evolution ofMicroorganisms · 2020Article
- Intracellular Redox Perturbation inFrontiers in bioengineering and biotechnology · 2020Article
- A microbubble-sparged yeast propagation-fermentation process for bioethanol production.Biotechnology for biofuels · 2020Article
- Evaluation of divergent yeast genera for fermentation-associated stresses and identification of a robust sugarcane distillery waste isolateBiotechnology for biofuels · 2019Article
- Screening and Mutation ofIndian journal of microbiology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundClassical strain engineering methods often have limitations in altering multigenetic cellular phenotypes. Here we try to improve
resultsError-prone PCR was employed to engineer the subunit Rpb7 of RNAP II to improve yeast ethanol tolerance and production. Based on previous studies and the presumption that improved ethanol resistance would lead to enhanced ethanol production, we first isolated variant M1 with much improved resistance towards 8 and 10% ethanol. The ethanol titers of M1 was ~122 g/L (96.58% of the theoretical yield) under laboratory very high gravity (VHG) fermentation, 40% increase as compared to the control. DNA microarray assay showed that 369 genes had differential expression in M1 after 12 h VHG fermentation, which are involved in glycolysis, alcoholic fermentation, oxidative stress response, etc.
conclusionsThis is the first study to demonstrate the possibility of engineering eukaryotic RNAP to alter global transcription profile and improve strain phenotypes. Targeting subunit Rpb7 of RNAP II was able to bring differential expression in hundreds of genes in
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.