ArticlePlant methods2017
Building a multipurpose insertional mutant library for forward and reverse genetics in
Article in Plant methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- The mosaic of aquatic photorespiration: evolution, diversity, and global biogeochemical impacts.The New phytologist · 2026Review
- Comparative Proteomics of Plasma and Flagellar Membranes inInternational journal of molecular sciences · 2026Article
- Knock Down ofPlants (Basel, Switzerland) · 2025Article
- Unwrapping the Ciliary Coat: High-Resolution Structure and Function of the Ciliary Glycocalyx.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Genome editing in the green alga Chlamydomonas: past, present practice and future prospects.The Plant journal : for cell and molecular biology · 2025Review
- CFAP410 has a bimodular architecture with a conserved surface patch on its N-terminal leucine-rich repeat motif for binding interaction partners.Frontiers in cell and developmental biology · 2025Article
- Fine-tuning the N-glycosylation of recombinant human erythropoietin using Chlamydomonas reinhardtii mutants.Plant biotechnology journal · 2024Article
- Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities.Biotechnology for biofuels and bioproducts · 2024Review
- CRISPR/Cas9-based mutant library screening: the discovery of novel genes regulating immune responses in cotton and rice.Frontiers in plant science · 2024Review
- Initial ciliary assembly inMolecular biology of the cell · 2023Article
- Lithium-induced ciliary lengthening sparks Arp2/3 complex-dependent endocytosis.Molecular biology of the cell · 2023Article
- Chlamydomonas proteases: classification, phylogeny, and molecular mechanisms.Journal of experimental botany · 2021Article
- Heme-binding protein CYB5D1 is a radial spoke component required for coordinated ciliary beating.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The ChloroplastCells · 2021Review
- Identification and Characterization of Genes Encoding the Hydroxypyruvate Reductases inFrontiers in plant science · 2021Article
- Disease Modeling To Understand the Pathomechanisms of Human Genetic Kidney Disorders.Clinical journal of the American Society of Nephrology : CJASN · 2020Review
- Review
- Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020Review
- A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism.The Plant cell · 2019Review
- PACRG and FAP20 form the inner junction of axonemal doublet microtubules and regulate ciliary motility.Molecular biology of the cell · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe unicellular green alga,
resultsBy selecting a suitable parental strain, keeping individual mutants using the agar plate, and designing an insertion cassette-specific primer for library screening, we successfully generated and maintained ~150,000 insertional mutants of
conclusionIn summary, this library provides a multifunctional platform both for obtaining mutants of interested genes and for screening of mutants with specific phenotype.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.