ArticleScientific reports2017
Validation of reference genes for the normalization of the RT-qPCR gene expression of virulence genes of Erwinia amylovora in apple shoots.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 28 citations in OpenAlex.
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- Identification of a novel real-time PCR reference gene panel for EA.hy926 endothelial cells in different growth States and with DHA treatment.Scientific reports · 2026Article
- Selecting reference genes for RT-qPCR studies involving the presence of B chromosomes in Psalidodon (Characiformes, Characidae).Molecular biology reports · 2024Article
- Bacterial-fungal crosstalk is defined by a fungal lactone mycotoxin and its degradation by a bacterial lactonase.Applied and environmental microbiology · 2024Article
- Transcriptome-based identification and validation of reference genes for corm growth stages, different tissues, and drought stress in Taro (Colocasia esculenta).BMC plant biology · 2024Article
- Validation of reference gene stability for normalization of RT-qPCR in Phytophthora capsici Leonian during its interaction with Piper nigrum L.Scientific reports · 2024Article
- I Choose You: Selecting Accurate Reference Genes for qPCR Expression Analysis in Reproductive Tissues inBiomolecules · 2023Article
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- Validation of reference genes for use in untreated bovine fibroblasts.Scientific reports · 2021Article
- Virulence Genetics of an Erwinia amylovora Putative Polysaccharide Transporter Family Member.Journal of bacteriology · 2020Article
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- Validation of reference genes for the normalization of RT-qPCR gene expression in Acanthamoeba spp.Scientific reports · 2020Article
- mRNA extraction of Xanthomonas fragariae in strawberry and validation of reference genes for the RT-qPCR for study of bacterial gene expression.Molecular biology reports · 2019Article
- Stable reference genes for RT-qPCR analysis of gene expression in the Musa acuminata-Pseudocercospora musae interaction.Scientific reports · 2019Article
- Selection of reference genes for measuring the expression of aiiO in Ochrobactrum quorumnocens A44 using RT-qPCR.Scientific reports · 2019Article
- Transcriptomic studies and assessment of Yersinia pestis reference genes in various conditions.Scientific reports · 2019Article
- Selection and validation of reference genes for gene expression studies in Klebsiella pneumoniae using Reverse Transcription Quantitative real-time PCR.Scientific reports · 2018Article
- Comparative transcriptome analysis of a lowly virulent strain of Erwinia amylovora in shoots of two apple cultivars - susceptible and resistant to fire blight.BMC genomics · 2017Article
- Identification of reference genes for qRT-PCR in granulosa cells of healthy women and polycystic ovarian syndrome patients.Scientific reports · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
To study the expression of pathogenicity-related genes in Erwinia amylovora, seven candidate reference genes (ffh, glyA, gyrA, proC, pykA, recA, rpoB) were selected and validated with the following five different mathematic algorithms: geNorm, NormFinder, BestKeeper, the delta CT method and the RefFinder web-based tool. An overall comprehensive ranking output from each of the selected software programs revealed that proC and recA, followed by ffh and pykA, were the most stably expressed genes and can be recommended for the normalization of RT-qPCR data. A combination of the three reference genes, proC, recA and ffh, allowed for the accurate expression analysis of amsB and hrpN genes and the calculation of their fold change in E. amylovora after its infection of susceptible and resistant apple cultivars. To the best of our knowledge, this is the first study presenting a list of the most suitable reference genes for use in the relative quantification of target gene expression in E. amylovora in planta, selected on the basis of a multi-algorithm analysis.
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