ArticleScientific reports2017
Microfluidic Sorting of Cells by Viability Based on Differences in Cell Stiffness.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 78 citations in OpenAlex.
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- Accessing the DNA in Breast Tissue: Adhesive Cell Capture from Flow onto Fibers of Cellulose Papers.ACS applied materials & interfaces · 2026Article
- High-throughput Genome Wide CRISPR Knock Out mechanical sort identifies genes driving metastatic cancer cell softening.bioRxiv : the preprint server for biology · 2026Article
- Target cell cortical tension regulates macrophage trogocytosis.Nature cell biology · 2025Article
- Dynamic and precise electromagnetic levitation of single cells.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Integration of acoustic, optical, and electrical methods in picoliter droplet microfluidics for rare particles enrichment.Communications engineering · 2025Article
- Controlling fluid flow rate to separate leukocytes and cancer cells based on stiffness differences.Mikrochimica acta · 2025Article
- Characterization, Enrichment, and Computational Modeling of Cross-Linked Actin Networks in Transformed Trabecular Meshwork Cells.Investigative ophthalmology & visual science · 2025Article
- Characterization, enrichment, and computational modeling of cross-linked actin networks in trabecular meshwork cells.bioRxiv : the preprint server for biology · 2024Article
- Label-free microfluidic isolation of functional and viable lymphocytes from peripheral blood mononuclear cells.Biomicrofluidics · 2023Article
- Continuous Flow Separation of Live and Dead Cells Using Gravity Sedimentation.Micromachines · 2023Article
- Advances in Microfluidics for Single Red Blood Cell Analysis.Biosensors · 2023Review
- Sensor technologies for quality control in engineered tissue manufacturing.Biofabrication · 2022Review
- Lessons from early life: understanding development to expand stem cells and treat cancers.Development (Cambridge, England) · 2022Review
- Scaling microfluidic throughput with flow-balanced manifolds to simply control devices with multiple inlets and outlets.Biomicrofluidics · 2022Article
- The role of physical cues in the development of stem cell-derived organoids.European biophysics journal : EBJ · 2022Review
- ViaChip for Size-based Enrichment of Viable Cells.Sensors and actuators. B, Chemical · 2022Article
- In vivo imaging of nanoparticle-labeled CAR T cells.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Massively parallel quantification of phenotypic heterogeneity in single-cell drug responses.Science advances · 2021Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
Abstract
The enrichment of viable cells is an essential step to obtain effective products for cell therapy. While procedures exist to characterize the viability of cells, most methods to exclude nonviable cells require the use of density gradient centrifugation or antibody-based cell sorting with molecular labels of cell viability. We report a label-free microfluidic technique to separate live and dead cells that exploits differences in cellular stiffness. The device uses a channel with repeated ridges that are diagonal with respect to the direction of cell flow. Stiff nonviable cells directed through the channel are compressed and translated orthogonally to the channel length, while soft live cells follow hydrodynamic flow. As a proof of concept, Jurkat cells are enriched to high purity of viable cells by a factor of 185-fold. Cell stiffness was validated as a sorting parameter as nonviable cells were substantially stiffer than live cells. To highlight the utility for hematopoietic stem cell transplantation, frozen samples of cord blood were thawed and the purity of viable nucleated cells was increased from 65% to over 94% with a recovery of 73% of the viable cells. Thus, the microfluidic stiffness sorting can simply and efficiently obtain highly pure populations of viable cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.