Evidence map›Paper›PMID 28515389›Full record

ArticleMicrobes and environments2017

Ca

Shin Haruta, Takao Iino, Moriya Ohkuma, Ken-Ichiro Suzuki, Yasuo Igarashi

Open access · diamondAbstract read
In one paragraph

Article in Microbes and environments, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 2 countries.

Shin HarutaDepartment of Biological Sciences, Tokyo Metropolitan University.
Takao IinoJapan Collection of Microorganisms, RIKEN BioResource Center.
Moriya OhkumaJapan Collection of Microorganisms, RIKEN BioResource Center.
Ken-Ichiro SuzukiNITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE).
Yasuo IgarashiGraduate School of Agricultural and Life Sciences, The University of Tokyo.
National Institute of Technology and Evaluation · JPRIKEN BioResource Research Center · JPSouthwest University · CNTokyo Metropolitan University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluorescence in situ hybridization (FISH) has been employed to identify microorganisms at the single cell level under a microscope. Extensive efforts have been made to improve and extend the FISH technique; however, the development of a widely applicable protocol is a continuing challenge. The present study evaluated the effects of divalent cations in the hybridization solution on the FISH-based detection of various species of bacteria and archaea with rRNA-targeted probes. A flow cytometric analysis after FISH with a standard hybridization buffer detected positive signals from less than 30% of Escherichia coli IAM 1264 cells. However, the number of cells with positive signals increased to more than 90% after the addition of calcium chloride to the hybridization buffer. Mn

Indexed as

In Situ Hybridization, FluorescenceCalciumEnterobacteriaceaeOligonucleotide ProbesCalciumOligonucleotide Probescalcium ionsEnterobacteriaceaeEscherichia coliflow cytometryfluorescence in situ hybridization

Identifiers

PMID28515389
PMCPMC5478537
OpenAlexW2615624894

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.