Evidence map›Paper›PMID 28515293›Full record

ArticleJournal of virology2017

Virus-Like Vesicles of Kaposi's Sarcoma-Associated Herpesvirus Activate Lytic Replication by Triggering Differentiation Signaling.

Danyang Gong, Xinghong Dai, Yuchen Xiao, Yushen Du, Travis J Chapa, Jeffrey R Johnson, Xinmin Li, Nevan J Krogan, Hongyu Deng, Ting-Ting Wu and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 1 pooled it
1.3field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it, 21 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Danyang GongDepartment of Molecular and Medical Pharmacology, University of California-Los Angeles, Los Angeles, California, USA.
Xinghong DaiDepartment of Molecular and Medical Pharmacology, University of California-Los Angeles, Los Angeles, California, USA.
Yuchen XiaoDepartment of Microbiology, Immunology, and Molecular Genetics, University of California-Los Angeles, Los Angeles, California, USA.
Yushen DuDepartment of Molecular and Medical Pharmacology, University of California-Los Angeles, Los Angeles, California, USA.
Travis J ChapaDepartment of Molecular and Medical Pharmacology, University of California-Los Angeles, Los Angeles, California, USA.
Jeffrey R JohnsonDepartment of Cellular and Molecular Pharmacology, University of California-San Francisco, San Francisco, California, USA.
Xinmin LiPathology and Laboratory Medicine, University of California-Los Angeles, Los Angeles, California, USA.
Nevan J KroganDepartment of Cellular and Molecular Pharmacology, University of California-San Francisco, San Francisco, California, USA.
Hongyu DengCAS Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing, People's Republic of China.
Ting-Ting WuDepartment of Molecular and Medical Pharmacology, University of California-Los Angeles, Los Angeles, California, USA.
Ren SunDepartment of Molecular and Medical Pharmacology, University of California-Los Angeles, Los Angeles, California, USA rsun@mednet.ucla.edu.
University of California, Los Angeles · USUniversity of California, San Francisco · USChinese Academy of Sciences · CN

Funding

Virology CoreP01CA177322 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WU, TING-TING · 2014 to 2018
$8.7M
Reactivation of Kaposi's Sarcoma-Associated HerpesvirusR01CA091791 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SUN, REN · 2001 to 2017
$4.4M
Develop a therapeutic vaccine approach by removing viral immune evasionR01DE023591 · NIDCR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SUN, REN · 2013 to 2017
$1.9M
NCI NIH HHS P01 CA177322NCI NIH HHS R01 CA091791NIDCR NIH HHS R01 DE023591
6 · The paper itself

Abstract

Virus-like vesicles (VLVs) are membrane-enclosed vesicles that resemble native enveloped viruses in organization but lack the viral capsid and genome. During the productive infection of tumor-associated gammaherpesviruses, both virions and VLVs are produced and are released into the extracellular space. However, studies of gammaherpesvirus-associated VLVs have been largely restricted by the technical difficulty of separating VLVs from mature virions. Here we report a strategy of selectively isolating VLVs by using a Kaposi's sarcoma-associated herpesvirus (KSHV) mutant that is defective in small capsid protein and is unable to produce mature virions. Using mass spectrometry analysis, we found that VLVs contained viral glycoproteins required for cellular entry, as well as tegument proteins involved in regulating lytic replication, but lacked capsid proteins. Functional analysis showed that VLVs induced the expression of the viral lytic activator RTA, initiating KSHV lytic gene expression. Furthermore, employing RNA sequencing, we performed a genomewide analysis of cellular responses triggered by VLVs and found that PRDM1, a master regulator in cell differentiation, was significantly upregulated. In the context of KSHV replication, we demonstrated that VLV-induced upregulation of PRDM1 was necessary and sufficient to reactivate KSHV by activating its RTA promoter. In sum, our study systematically examined the composition of VLVs and demonstrated their biological roles in manipulating host cell responses and facilitating KSHV lytic replication.

Indexed as

Gene Expression Regulation, ViralHost-Pathogen InteractionsSignal TransductionVirus ReplicationCell DifferentiationCell LineHerpesvirus 8, HumanHumansImmediate-Early ProteinsPositive Regulatory Domain I-Binding Factor 1Repressor ProteinsTrans-ActivatorsUp-RegulationVirosomesImmediate-Early ProteinsPositive Regulatory Domain I-Binding Factor 1PRDM1 protein, humanRepressor ProteinsRta protein, Human herpesvirus 8Trans-ActivatorsVirosomesEBVenvelope proteinextracellular vesicleherpesvirusKSHVmicroRNAPRDM1RTAtegument proteinvirus-like vesicle

Identifiers

PMID28515293
PMCPMC5651724
OpenAlexW2614707402

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.