ArticleNature2017
Identification of preoptic sleep neurons using retrograde labelling and gene profiling.
Article in Nature, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 182 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
182 citing papers in PubMed, 1 synthesis or guideline pooled it, 321 citations in OpenAlex.
- Effects of non-pharmacological interventions on sleep in patients with critical illness: a systematic review and network meta-analysis.Scientific reports · 2026Pooled it
- A phase 1 double-blind, placebo-controlled study of zuranolone (SAGE-217) in a phase advance model of insomnia in healthy adults.Human psychopharmacology · 2022Trial
- A corticohypothalamic circuit that mediates maternal awakening to pup cries.Nature neuroscience · 2026Article
- Article
- A male-biased hypothalamic circuit modulates postmating sleep suppression in mice.Science advances · 2026Article
- Delta-Band Dynamics of CaMKI-Positive Preoptic Neurons Encode Thermal Homeostatic Information.Neuroscience bulletin · 2026Article
- Sleep circuit modulation: from animal models to human translation.Brain : a journal of neurology · 2026Review
- Article
- Single-Nucleus Transcriptomics of the Mouse Medial Preoptic Area Reveals Sex-Dependent Molecular Signatures of Social Dominance.bioRxiv : the preprint server for biology · 2026Article
- JACUZI-SD: An automated, high-throughput, minimally stressful approach to sleep depriving larval zebrafish.iScience · 2026Article
- Role of Preoptic Area γ-Aminobutyric Acid-mediated Neurons in Distinctly Regulating Sleep and Rousability during Dexmedetomidine Sedation in Mice.Anesthesiology · 2026Article
- Article
- Insomnia: the gut microbiome connection, prospects for probiotic and postbiotic therapies, and future directions.Journal of advanced research · 2026Review
- Impact of NPAS2 on mPFC dopamine synthesis and nap behavior.Nature communications · 2026Article
- Disrupted temperature-sleep coupling mechanism in a Dravet syndrome mouse model.Nature communications · 2026Article
- Neurotensin in the extended amygdala maintains wakefulness in novel environments.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Stress ensemble in the BNST mediates comorbid anxiety and sleep disruption.Science advances · 2026Article
- Article
- The ventrolateral preoptic nucleus: a multisystem integrator in sleep-wake regulation.Frontiers in neuroscience · 2026Review
- Neural Circuit Mechanisms of General Anaesthesia Induction: Current Advances and Future Directions.Current neuropharmacology · 2026Review
122 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
18 authors at 6 institutions in 1 country.
Funding
Abstract
In humans and other mammalian species, lesions in the preoptic area of the hypothalamus cause profound sleep impairment, indicating a crucial role of the preoptic area in sleep generation. However, the underlying circuit mechanism remains poorly understood. Electrophysiological recordings and c-Fos immunohistochemistry have shown the existence of sleep-active neurons in the preoptic area, especially in the ventrolateral preoptic area and median preoptic nucleus. Pharmacogenetic activation of c-Fos-labelled sleep-active neurons has been shown to induce sleep. However, the sleep-active neurons are spatially intermingled with wake-active neurons, making it difficult to target the sleep neurons specifically for circuit analysis. Here we identify a population of preoptic area sleep neurons on the basis of their projection target and discover their molecular markers. Using a lentivirus expressing channelrhodopsin-2 or a light-activated chloride channel for retrograde labelling, bidirectional optogenetic manipulation, and optrode recording, we show that the preoptic area GABAergic neurons projecting to the tuberomammillary nucleus are both sleep active and sleep promoting. Furthermore, translating ribosome affinity purification and single-cell RNA sequencing identify candidate markers for these neurons, and optogenetic and pharmacogenetic manipulations demonstrate that several peptide markers (cholecystokinin, corticotropin-releasing hormone, and tachykinin 1) label sleep-promoting neurons. Together, these findings provide easy genetic access to sleep-promoting preoptic area neurons and a valuable entry point for dissecting the sleep control circuit.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.