Evidence map›Paper›PMID 28513556›Full record

ArticleMetabolites2017

Microscale Quantitative Analysis of Polyhydroxybutyrate in Prokaryotes Using IDMS.

Mariana Itzel Velasco Alvarez, Angela Ten Pierick, Patricia T N van Dam, Reza Maleki Seifar, Mark C M van Loosdrecht, S Aljoscha Wahl

Open access · goldAbstract read
In one paragraph

Article in Metabolites, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Mariana Itzel Velasco AlvarezCell Systems Engineering Group, Department of Biotechnology, Delft University of Technology, 2629 HZ Delft, The Netherlands. M.I.VelascoAlvarez@tudelft.nl.
Angela Ten PierickBioAnalytical Chemistry, Department of Chemistry and Pharmaceutical Sciences, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands. a.ten.pierick@vu.nl.
Patricia T N van DamCell Systems Engineering Group, Department of Biotechnology, Delft University of Technology, 2629 HZ Delft, The Netherlands. P.T.N.vanDam@tudelft.nl.
Reza Maleki SeifarCell Systems Engineering Group, Department of Biotechnology, Delft University of Technology, 2629 HZ Delft, The Netherlands. Reza.Maleki-Seifar@dsm.com.
Mark C M van LoosdrechtCell Systems Engineering Group, Department of Biotechnology, Delft University of Technology, 2629 HZ Delft, The Netherlands. M.C.M.vanLoosdrecht@tudelft.nl.
S Aljoscha WahlCell Systems Engineering Group, Department of Biotechnology, Delft University of Technology, 2629 HZ Delft, The Netherlands. s.a.wahl@tudelft.nl.
Delft University of Technology · NLVrije Universiteit Amsterdam · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Poly(3-hydroxybutyrate) (PHB) is an interesting biopolymer for replacing petroleum-based plastics, its biological production is performed in natural and engineered microorganisms. Current metabolic engineering approaches rely on high-throughput strain construction and screening. Analytical procedures have to be compatible with the small scale and speed of these approaches. Here, we present a method based on isotope dilution mass spectrometry (IDMS) and propanolysis extraction of poly(3-hydroxybutyrate) from an

Indexed as

13C-labeled internal standard3-hydroxy butyrateEscherichia coliGC-MSisotope dilutionpolyhydroxybutyrate

Identifiers

PMID28513556
PMCPMC5487990
OpenAlexW2614628142

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.