ArticleUpsala journal of medical sciences2017
Molecular composition and distribution of gap junctions in the sensory epithelium of the human cochlea-a super-resolution structured illumination microscopy (SR-SIM) study.
Article in Upsala journal of medical sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 28 citations in OpenAlex.
- An Ala/Glu difference in E1 of Cx26 and Cx30 contributes to their differential anionic permeabilities.The Journal of general physiology · 2024Article
- A pore locus in the E1 domain differentially regulates Cx26 and Cx30 hemichannel function.The Journal of general physiology · 2024Article
- Microanatomy of the human tunnel of Corti structures and cochlear partition-tonotopic variations and transcellular signaling.Journal of anatomy · 2024Article
- Abnormal Innervation, Demyelination, and Degeneration of Spiral Ganglion Neurons as Well as Disruption of Heminodes are Involved in the Onset of Deafness in Cx26 Null Mice.Neuroscience bulletin · 2024Article
- Connexin Mutations and Hereditary Diseases.International journal of molecular sciences · 2022Review
- Age-Related Inflammation and Oxidative Stress in the Cochlea Are Exacerbated by Long-Term, Short-Duration Noise Stimulation.Frontiers in aging neuroscience · 2022Article
- Article
- A combined genome-wide association and molecular study of age-related hearing loss in H. sapiens.BMC medicine · 2021Article
- Two Photon Fluorescence Microscopy of the Unstained Human Cochlea Reveals Organ of Corti Cytoarchitecture.Frontiers in cellular neuroscience · 2021Article
- Practical aspects of inner ear gene delivery for research and clinical applications.Hearing research · 2020Review
- Expression of Na/K-ATPase subunits in the human cochlea: a confocal and super-resolution microscopy study with special reference to auditory nerve excitation and cochlear implantation.Upsala journal of medical sciences · 2019Article
- Inner Ear Connexin Channels: Roles in Development and Maintenance of Cochlear Function.Cold Spring Harbor perspectives in medicine · 2019Review
- Human Inner Ear Immune Activity: A Super-Resolution Immunohistochemistry Study.Frontiers in neurology · 2019Article
- The spatial distribution pattern of Connexin26 expression in supporting cells and its role in outer hair cell survival.Cell death & disease · 2018Article
- Human inner ear blood supply revisited: the Uppsala collection of temporal bone-an international resource of education and collaboration.Upsala journal of medical sciences · 2018Article
- Expression of trans-membrane serine protease 3 (TMPRSS3) in the human organ of Corti.Cell and tissue research · 2018Article
- The secondary spiral lamina and its relevance in cochlear implant surgery.Upsala journal of medical sciences · 2018Article
- Bridging the gap: Super-resolution microscopy of epithelial cell junctions.Tissue barriers · 2018Review
- The Human Endolymphatic Sac and Inner Ear Immunity: Macrophage Interaction and Molecular Expression.Frontiers in immunology · 2018Article
- Macrophages in the Human Cochlea: Saviors or Predators-A Study Using Super-Resolution Immunohistochemistry.Frontiers in immunology · 2018Article
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMutations in the GJB2 gene, which encodes the Connexin26 (Cx26) protein, are the most common cause of childhood hearing loss in American and European populations. The cochlea contains a gap junction (GJ) network in the sensory epithelium and two connective tissue networks in the lateral wall and spiral limbus. The syncytia contain the GJ proteins beta 2 (GJB2/Cx26) and beta 6 (GJB6/Cx30). Our knowledge of their expression in humans is insufficient due to the limited availability of tissue. Here, we sought to establish the molecular arrangement of GJs in the epithelial network of the human cochlea using surgically obtained samples.
methodsWe analyzed Cx26 and Cx30 expression in GJ networks in well-preserved adult human auditory sensory epithelium using confocal, electron, and super-resolution structured illumination microscopy (SR-SIM).
resultsCx30 plaques (<5 μm) dominated, while Cx26 plaques were subtle and appeared as 'mini-junctions' (2-300 nm). 3-D volume rendering of Z-stacks and orthogonal projections from single optical sections suggested that the GJs are homomeric/homotypic and consist of assemblies of identical GJs composed of either Cx26 or Cx30. Occasionally, the two protein types were co-expressed, suggesting functional cooperation.
conclusionsEstablishing the molecular composition and distribution of the GJ networks in the human cochlea may increase our understanding of the pathophysiology of Cx-related hearing loss. This information may also assist in developing future strategies to treat genetic hearing loss.
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