ArticleBotanical studies2014
Application of an inducible transposon with anther culture in generation of di-haploid homologous mutants.
Article in Botanical studies, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Embryo Rescue in Plant Breeding.Plants (Basel, Switzerland) · 2023Review
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2 authors.
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Abstract
backgroundInsertional mutagenesis represents one of the most effective ways to acquire information about a plant gene's function. However, it is hindered by the autosomal genome being diploid and therefore, most mutations being recessive. The problem is addressed by inducing the transposition during anther culture so that selected mutations can be transmitted and then regenerated to a homozygous state.
resultsTo this end, we treated transgenic rice floral tissues containing the inducible transposon with an inducer, salicylic acid. Excision events were detected in regenerated calli and subsequent plantlets. DNA blot and PCR assay were used to determine the homogeneity of knockout mutants. About 5% of the mutants containing transposition events were homozygous. Furthermore, the inducible transposon was active during calli regeneration.
conclusionsThis strategy could be applicable to improve transposition efficiency in microspore development stages to create stable di-haploid mutants in plants.
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