ArticleBotanical studies2013
Tissue culture-induced somaclonal variation of decreased pollen viability in torenia (Torenia fournieri Lind.).
Article in Botanical studies, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 42 citations in OpenAlex.
- A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts.Plants (Basel, Switzerland) · 2021Article
- Barley somatic embryogenesis-an attempt to modify variation induced in tissue culture.Journal of biological research (Thessalonike, Greece) · 2021Article
- Medical Cannabis and Industrial Hemp Tissue Culture: Present Status and Future Potential.Frontiers in plant science · 2021Review
- Genetic and Global Epigenetic Modification, Which Determines the Phenotype of Transgenic Rice?International journal of molecular sciences · 2020Article
- Review
- Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation.Plant cell reports · 2016Article
- The biology and in vitro propagation of the ornamental aquatic plant,SpringerPlus · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
backgroundPhenotypic and genotypic variations, collectively called somaclonal variations, are induced during tissue culture.
resultsWe studied the phenotypic variation in pollen viability of regenerants of torenia after subculturing for one to nine generations. We found that pollen viability of regenerants continuously decreased with increasing subculture time. High concentrations of plant growth regulators applied to the Murashige and Skoog (MS) medium also resulted in diminished pollen viability. Furthermore, antibiotic application during gene transformation also decreased pollen viability of the transformants. However, the process of long-term culture did not significantly change pollen viability. The mean methylation level of regenerants showed a 0.28% to 3.95% decrease in seedlings subcultured in vitro for nine generations. Moreover, when the ninth subcultured regenerants with reduced pollen vibility were recovered in soil to get seeds, the pollen viability of seed-derive plants was similar to that of the wild type.
conclusionsThe results show that plant growth regulators, antibiotics, and the number of subculture generations influence somaclonal variations in torenia. The somaclonal variations in torenia may results from epigenetic changes.
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