ArticlePLoS neglected tropical diseases2017
The Biomphalaria glabrata DNA methylation machinery displays spatial tissue expression, is differentially active in distinct snail populations and is modulated by interactions with Schistosoma mansoni.
Article in PLoS neglected tropical diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 65 citations in OpenAlex.
- Article
- Epigenetic and metabolic reprogramming of innate immune cells establishes immunological memory in the Schistosomiasis vector snail Biomphalaria glabrata.PLoS pathogens · 2026Article
- Use of a cytochrome P450 humanized mouse model to refine schistosomiasis drug discovery.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Novel 1ACS infectious diseases · 2026Article
- A systems-level analysis of global gene expression in resistant and susceptibleFrontiers in immunology · 2026Article
- Introduction of epigenetic variation contribute to resistance against the human parasite Schistosoma mansoni.Epigenetics & chromatin · 2025Article
- Article
- DNA Methylation Machinery in Gastropod Mollusks.Life (Basel, Switzerland) · 2024Review
- Using ChEMBL to Complement Schistosome Drug Discovery.Pharmaceutics · 2023Article
- Pulmonary inflammation promoted by type-2 dendritic cells is a feature of human and murine schistosomiasis.Nature communications · 2023Article
- Chemical modulation ofWellcome open research · 2023Article
- The mechanisms and factors that induce trained immunity in arthropods and mollusks.Frontiers in immunology · 2023Review
- Cadmium nitrate and DNA methylation in gastropods: comparison between ovotestis and hepatopancreas.PeerJ · 2023Article
- Nuclear genome of Bulinus truncatus, an intermediate host of the carcinogenic human blood fluke Schistosoma haematobium.Nature communications · 2022Article
- Hit-and-Run Epigenetic Editing for Vectors of Snail-Borne Parasitic Diseases.Frontiers in cell and developmental biology · 2022Review
- Anti-schistosomal activities of quinoxaline-containing compounds: From hit identification to lead optimisation.European journal of medicinal chemistry · 2021Article
- The methylome of Biomphalaria glabrata and other mollusks: enduring modification of epigenetic landscape and phenotypic traits by a new DNA methylation inhibitor.Epigenetics & chromatin · 2021Article
- DNA methylation atlas and machinery in the developing and regenerating annelid Platynereis dumerilii.BMC biology · 2021Article
- Inference of DNA methylation patterns in molluscs.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Article
- Structural Requirements for Dihydrobenzoxazepinone Anthelmintics: Actions against Medically Important and Model Parasites:ACS infectious diseases · 2021Article
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Authors and funding
13 authors at 4 institutions in 4 countries.
Funding
Abstract
backgroundThe debilitating human disease schistosomiasis is caused by infection with schistosome parasites that maintain a complex lifecycle alternating between definitive (human) and intermediate (snail) hosts. While much is known about how the definitive host responds to schistosome infection, there is comparably less information available describing the snail's response to infection. METHODOLOGY/PRINCIPLE
findingsHere, using information recently revealed by sequencing of the Biomphalaria glabrata intermediate host genome, we provide evidence that the predicted core snail DNA methylation machinery components are associated with both intra-species reproduction processes and inter-species interactions. Firstly, methyl-CpG binding domain protein (Bgmbd2/3) and DNA methyltransferase 1 (Bgdnmt1) genes are transcriptionally enriched in gonadal compared to somatic tissues with 5-azacytidine (5-AzaC) treatment significantly inhibiting oviposition. Secondly, elevated levels of 5-methyl cytosine (5mC), DNA methyltransferase activity and 5mC binding in pigmented hybrid- compared to inbred (NMRI)- B. glabrata populations indicate a role for the snail's DNA methylation machinery in maintaining hybrid vigour or heterosis. Thirdly, locus-specific detection of 5mC by bisulfite (BS)-PCR revealed 5mC within an exonic region of a housekeeping protein-coding gene (Bg14-3-3), supporting previous in silico predictions and whole genome BS-Seq analysis of this species' genome. Finally, we provide preliminary evidence for parasite-mediated host epigenetic reprogramming in the schistosome/snail system, as demonstrated by the increase in Bgdnmt1 and Bgmbd2/3 transcript abundance following Bge (B. glabrata embryonic cell line) exposure to parasite larval transformation products (LTP). CONCLUSIONS/SIGNIFICANCE: The presence of a functional DNA methylation machinery in B. glabrata as well as the modulation of these gene products in response to schistosome products, suggests a vital role for DNA methylation during snail development/oviposition and parasite interactions. Further deciphering the role of this epigenetic process during Biomphalaria/Schistosoma co-evolutionary biology may reveal key factors associated with disease transmission and, moreover, enable the discovery of novel lifecycle intervention strategies.
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