Evidence map›Paper›PMID 28510359›Full record

ArticleCurrent protocols in microbiology2017

Isolation, Culture and Cryopreservation of Sarcocystis species.

S K Verma, D S Lindsay, M E Grigg, J P Dubey

Abstract read
In one paragraph

Article in Current protocols in microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed.

  1. Article
  2. Pathogens (Basel, Switzerland) · 2026
    Article
  3. Article
  4. Molecular and Microscopic Identification ofAnimals : an open access journal from MDPI · 2026
    Article
  5. Article
  6. Article
  7. Article
  8. Molecular Screening ofAnimals : an open access journal from MDPI · 2025
    Article
  9. European Pine Marten (Veterinary sciences · 2025
    Article
  10. Molecular Evidence of Raccoon DogPathogens (Basel, Switzerland) · 2025
    Article
  11. Article
  12. Article
  13. Investigation of the parasiteOpen veterinary journal · 2025
    Article
  14. Article
  15. The Genetic Identification of Numerous ApicomplexanAnimals : an open access journal from MDPI · 2024
    Article
  16. Article
  17. Identification ofVeterinary sciences · 2024
    Article
  18. Article
  19. Molecular Confirmation ofPathogens (Basel, Switzerland) · 2023
    Article
  20. Molecular Identification of ProtozoanLife (Basel, Switzerland) · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

S K VermaUnited States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, Maryland.
D S LindsayDepartment of Biomedical Science and Pathology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia.
M E GriggMolecular Parasitology Section, Laboratory of Parasitic Diseases, National Institutes of Health, National Institutes of Allergy, and Infectious Diseases, Bethesda, Maryland.
J P DubeyUnited States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, Maryland.

Funding

Toxoplasma Population GeneticsZIAAI001018 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI GRIGG, MICHAEL · 2009 to 2025
$11.3M
Intramural NIH HHS ZIA AI001018
6 · The paper itself

Abstract

More than 200 valid Sarcocystis species have been described in the parasitological literature. The developmental life cycle in the intermediate host and definitive host has only been described for a few species. Sarcocystis parasites are common pathogens infecting a wide range of animals, including humans, and this unit reviews the methods used for isolating infective stages of the parasite from both definitive and intermediate host(s), as well as methods used to initiate cultures from sporocysts and merozoites and for cryopreservation of various Sarcocystis spp. These methods are based on published reports and our experience with Sarcocystis species in cell culture over many years. The information presented is suitable for the efficient culture of many Sarcocystis species; however, some minor modifications may be needed based on the unique developmental patterns of some species. © 2017 by John Wiley & Sons, Inc.

Indexed as

AnimalsCryopreservationHumansParasitologySarcocystiscryopreservationculturehostisolationparasiteSarcocystis

Identifiers

PMID28510359
PMCPMC5570482

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.