ArticleInternational journal of molecular sciences2017
Analysis of Different Approaches for the Selection of Reference Genes in RT-qPCR Experiments: A Case Study in Skeletal Muscle of Growing Mice.
Article in International journal of molecular sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- A systematic review on the selection of reference genes for gene expression studies in rodents: are the classics the best choice?Molecular biology reports · 2024Pooled it
- Subcutaneous Infusion of DNA-Aptamer Raised against Advanced Glycation End Products Prevents Loss of Skeletal Muscle Mass and Strength in Accelerated-Aging Mice.Biomedicines · 2023Article
- Article
- Selection of Ideal Reference Genes for Gene Expression Analysis in COVID-19 and Mucormycosis.Microbiology spectrum · 2022Article
- Evaluation of Reference Genes for Real-Time Quantitative PCR Analysis in Tissues from Bumble Bees (International journal of molecular sciences · 2022Article
- Insulin signaling in the heart is impaired by growth hormone: a direct and early event.Journal of molecular endocrinology · 2022Article
- Comparison of reference gene expression stability in mouse skeletal muscle via five algorithms.PeerJ · 2022Article
- Genome-Wide Identification of Reference Genes for Reverse-Transcription Quantitative PCR in Goat Rumen.Animals : an open access journal from MDPI · 2021Article
- Screening Potential Reference Genes inGenes · 2021Article
- Force dependent effects of chronic overuse on fibrosis-related genes and proteins in skeletal muscles.Connective tissue research · 2021Article
- A rapid RNA extraction method from oil palm tissues suitable for reverse transcription quantitative real-time PCR (RT-qPCR).3 Biotech · 2020Article
- Roles of p38α and p38β mitogen‑activated protein kinase isoforms in human malignant melanoma A375 cells.International journal of molecular medicine · 2019Article
- Validation of Common Housekeeping Genes as Reference for qPCR Gene Expression Analysis During iPS Reprogramming Process.Scientific reports · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
Abstract
The reliability of reverse transcription-quantitative PCR (RT-qPCR) results in gene expression studies depends on the approaches used to account for non-biological variations. In order to find a proper normalization strategy for the study of genes related to growth hormone signaling in skeletal muscle of growing mice, nine unrelated genes were evaluated as internal controls. According to the most used algorithms-geNorm, the Comparative Δ
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.