ArticleSensors (Basel, Switzerland)2017
Biosensor Based on Tyrosinase Immobilized on Graphene-Decorated Gold Nanoparticle/Chitosan for Phenolic Detection in Aqueous.
Article in Sensors (Basel, Switzerland), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 74 citations in OpenAlex.
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- Ultrasensitive detection of aromatic water pollutants through protein immobilization driven organic electrochemical transistors.Chemical science · 2024Article
- Tyrosinase Immobilization Strategies for the Development of Electrochemical Biosensors-A Review.Nanomaterials (Basel, Switzerland) · 2023Review
- A Catechol-Meter Based on Conventional Personal Glucose Meter for Portable Detection of Tyrosinase and Sodium Benzoate.Biosensors · 2022Article
- Novel Amperometric Biosensor Based on Tyrosinase/Chitosan Nanoparticles for Sensitive and Interference-Free Detection of Total Catecholamine.Biosensors · 2022Article
- Synthesis, Toxicity Assessment, Environmental and Biomedical Applications of MXenes: A Review.Nanomaterials (Basel, Switzerland) · 2022Review
- A Ready-to-Use Metal-Supported Bilayer Lipid Membrane Biosensor for the Detection of Phenol in Water.Membranes · 2021Article
- Recent Advances in Chitin and Chitosan/Graphene-Based Bio-Nanocomposites for Energetic Applications.Polymers · 2021Review
- Silver Nanowires as Electron Transfer Mediators in Electrochemical Catechol Biosensors.Sensors (Basel, Switzerland) · 2021Article
- Pickering-emulsion-templated synthesis of 3D hollow graphene as an efficient oil absorbent.RSC advances · 2021Article
- Potentiometric polyphenol oxidase biosensor for sensitive determination of phenolic micropollutant in environmental samples.Environmental science and pollution research international · 2020Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this research work, electrochemical biosensor was fabricated based on immobilization of tyrosinase onto graphene-decorated gold nanoparticle/chitosan (Gr-Au-Chit/Tyr) nanocomposite-modified screen-printed carbon electrode (SPCE) for the detection of phenolic compounds. The nanocomposite film was constructed via solution casting method. The electrocatalytic activity of the proposed biosensor for phenol detection was studied using differential pulse voltammetry (DPV) and cyclic voltammetry (CV). Experimental parameters such as pH buffer, enzyme concentration, ratio of Gr-Au-Chit, accumulation time and potential were optimized. The biosensor shows linearity towards phenol in the concentration range from 0.05 to 15 μM with sensitivity of 0.624 μA/μM and the limit of detection (LOD) of 0.016 μM (S/N = 3). The proposed sensor also depicts good reproducibility, selectivity and stability for at least one month. The biosensor was compared with high-performance liquid chromatography (HPLC) method for the detection of phenol spiked in real water samples and the result is in good agreement and comparable.
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Registered trials
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