ArticleNature communications2017
Protein-protein interactions and metabolite channelling in the plant tricarboxylic acid cycle.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 154 citations in OpenAlex.
- Dynamic assembly of malate dehydrogenase-citrate synthase multienzyme complex in the mitochondria.eLife · 2026Article
- Molecular architecture of the human citrate synthase-malate dehydrogenase 2 metabolon.Acta crystallographica. Section D, Structural biology · 2026Article
- Complete enzyme clustering enhances coenzyme Q biosynthesis via substrate channeling.Nature communications · 2026Article
- PERSPECTIVE: organelle positioning as a principle of metabolic regulation and stress tolerance.The Plant journal : for cell and molecular biology · 2026Review
- Mammalian-like steroidogenesis in plants gives rise to endocrine-mimetic cardenolides.Science advances · 2026Article
- Protein-Protein Interactions Modulate a Key Branch Point in Monoterpene Indole Alkaloid Biosynthesis.ACS chemical biology · 2026Article
- Synthetic crassulacean acid metabolism (SynCAM) for improving water-use efficiency in plants.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- RNA-binding proteins as versatile metabolic regulators.npj metabolic health and disease · 2025Article
- Article
- Perception and processing of stress signals by plant mitochondria.The Plant journal : for cell and molecular biology · 2024Review
- Elucidating the metabolic roles of isoflavone synthase-mediated protein-protein interactions in yeast.bioRxiv : the preprint server for biology · 2024Article
- Is the TCA cycle malate dehydrogenase-citrate synthase metabolon an illusion?Essays in biochemistry · 2024Review
- Biochemical and Epigenetic Regulation of Glutamate Metabolism in Maize (Plants (Basel, Switzerland) · 2024Article
- Accurate Long-Read RNA Sequencing Analysis Reveals the Key Pathways and Candidate Genes under Drought Stress in the Seed Germination Stage in Faba Bean.International journal of molecular sciences · 2024Article
- Elucidating the Role of Human ALAS2 C-terminal Mutations Resulting in Loss of Function and Disease.Biochemistry · 2024Article
- Non-consecutive enzyme interactions within TCA cycle supramolecular assembly regulate carbon-nitrogen metabolism.Nature communications · 2024Article
- Enhancing the Spermidine Synthase-Based Polyamine Biosynthetic Pathway to Boost Rapid Growth in Marine DiatomBiomolecules · 2024Article
- DIA-Based Quantitative Proteomics in the Flower Buds of TwoInternational journal of molecular sciences · 2024Article
- Exploring the Evolvability of Plant Specialized Metabolism: Uniqueness Out Of Uniformity and Uniqueness Behind Uniformity.Plant & cell physiology · 2023Review
- The conditional mitochondrial protein complexome in the Arabidopsis thaliana root and shoot.Plant communications · 2023Article
13 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein complexes of sequential metabolic enzymes, often termed metabolons, may permit direct channelling of metabolites between the enzymes, providing increased control over metabolic pathway fluxes. Experimental evidence supporting their existence in vivo remains fragmentary. In the present study, we test binary interactions of the proteins constituting the plant tricarboxylic acid (TCA) cycle. We integrate (semi-)quantitative results from affinity purification-mass spectrometry, split-luciferase and yeast-two-hybrid assays to generate a single reliability score for assessing protein-protein interactions. By this approach, we identify 158 interactions including those between catalytic subunits of sequential enzymes and between subunits of enzymes mediating non-adjacent reactions. We reveal channelling of citrate and fumarate in isolated potato mitochondria by isotope dilution experiments. These results provide evidence for a functional TCA cycle metabolon in plants, which we discuss in the context of contemporary understanding of this pathway in other kingdoms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.