Evidence map›Paper›PMID 28508570›Full record

ArticleImmunity, inflammation and disease2017

A new transcript in the TCRB locus unveils the human ortholog of the mouse pre-Dß1 promoter.

Bernard Lethé, Sylvia Snauwaert, Orian Bricard, David Schröder, Tiphanie Gomard, Gérald Hames, Catherine Muller, Christophe Lurquin, Emilie Gauthy, Ahmed Essaghir and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
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Article in Immunity, inflammation and disease, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Bernard LethéLudwig Institute for Cancer Research, Brussels, Belgium.
Sylvia SnauwaertDepartment of Clinical Chemistry, Microbiology and Immunology, Ghent University, Ghent, Belgium.
Orian Bricardde Duve Institute, Université catholique de Louvain, Brussels, Belgium.
David Schröderde Duve Institute, Université catholique de Louvain, Brussels, Belgium.
Tiphanie Gomardde Duve Institute, Université catholique de Louvain, Brussels, Belgium.
Gérald Hamesde Duve Institute, Université catholique de Louvain, Brussels, Belgium.
Catherine Mullerde Duve Institute, Université catholique de Louvain, Brussels, Belgium.
Christophe LurquinLudwig Institute for Cancer Research, Brussels, Belgium.
Emilie Gauthyde Duve Institute, Université catholique de Louvain, Brussels, Belgium.
Ahmed Essaghirde Duve Institute, Université catholique de Louvain, Brussels, Belgium.
Bart VandekerckhoveDepartment of Clinical Chemistry, Microbiology and Immunology, Ghent University, Ghent, Belgium.
Pierre G Couliede Duve Institute, Université catholique de Louvain, Brussels, Belgium.ORCID 0000-0003-2916-2938
de Duve Institute · BEGhent University Hospital · BELudwig Cancer Research · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionWhile most transcripts arising from the human T Cell Receptor locus reflect fully rearranged genes, several germline transcripts have been identified. We describe a new germline transcript arising from the human TCRB locus.

methodscDNA sequencing, promoter, and gene expression analyses were used to characterize the new transcript.

resultsThe new germline transcript encoded by the human TCRB locus consists of a new exon of 103 bp, which we named TRBX1 (X1), spliced with the first exon of gene segments Cß1 or Cß2. X1 is located upstream of gene segment Dß1 and is therefore deleted from a V-DJ rearranged TCRB locus. The X1-Cß transcripts do not appear to code for a protein. We define their transcription start and minimal promoter. These transcripts are found in populations of mature T lymphocytes from blood or tissues and in T cell clones with a monoallelic TCRB rearrangement. In immature thymocytes, they are already detectable in CD1a

conclusionsThe X1 promoter appears to be the ortholog of the mouse pre-Dß1 promoter (PDß1). Like PDß1, its activation is regulated by Eß in T cells and might facilitate the TCRB rearrangement process by contributing to the accessibility of the Dß1 locus.

Indexed as

Genes, T-Cell Receptor betaGenetic LociPromoter Regions, GeneticTranscription, GeneticAnimalsHumansMiceRNA, MessengerRNA, MessengerAllelic inclusionhuman TCRB locuslocus accessibilityPDß1 germline transcriptsT cellsTCRB transcriptsthymocytes

Identifiers

PMID28508570
PMCPMC5569374
OpenAlexW2616801604

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