Evidence map›Paper›PMID 28508553›Full record

ArticleProteomics2017

A targeted proteomic assay for the measurement of plasma proteoforms related to human aging phenotypes.

Richard D Semba, Pingbo Zhang, Min Zhu, Elisa Fabbri, Marta Gonzalez-Freire, Ruin Moaddel, Minghui Geng-Spyropoulos, Luigi Ferrucci

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proteomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Review
  6. Review
  7. Review
  8. Can Serum Nitrosoproteome Predict Longevity of Aged Women?International journal of molecular sciences · 2020
    Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Richard D SembaWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Pingbo ZhangWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Min ZhuNational Institute on Aging, National Institutes of Health, Baltimore, MD, USA.
Elisa FabbriNational Institute on Aging, National Institutes of Health, Baltimore, MD, USA.
Marta Gonzalez-FreireNational Institute on Aging, National Institutes of Health, Baltimore, MD, USA.
Ruin MoaddelNational Institute on Aging, National Institutes of Health, Baltimore, MD, USA.
Minghui Geng-SpyropoulosWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Luigi FerrucciNational Institute on Aging, National Institutes of Health, Baltimore, MD, USA.
National Institute on Aging · USJohns Hopkins University · USJohns Hopkins Medicine · USNational Institutes of Health · US

Funding

Oxidative stress and the pathogenesis of sarcopenia and disability in older womenR01AG027012 · NIA · JOHNS HOPKINS UNIVERSITY · PI SEMBA, RICHARD D · 2005 to 2018
$3.6M
Systemic complement and age-related macular degeneration.R01EY024596 · NEI · JOHNS HOPKINS UNIVERSITY · PI SEMBA, RICHARD D · 2014 to 2016
$1.6M
Relationship of candidate circulating proteoforms with aging phenotypes.R56AG052973 · NIA · JOHNS HOPKINS UNIVERSITY · PI SEMBA, RICHARD D · 2016 to 2016
$211k
Intramural NIH HHS Z99 AG999999NEI NIH HHS R01 EY024596NIA NIH HHS R01 AG027012NIA NIH HHS R56 AG052973
6 · The paper itself

Abstract

Circulating polypeptides and proteins have been implicated in reversing or accelerating aging phenotypes, including growth/differentiation factor 8 (GDF8), GDF11, eotaxin, and oxytocin. These proteoforms, which are defined as the protein products arising from a single gene due to alternative splicing and PTMs, have been challenging to study. Both GDF8 and GDF11 have known antagonists such as follistatin (FST), and WAP, Kazal, immunoglobulin, Kunitz, and NTR domain-containing proteins 1 and 2 (WFIKKN1, WFIKKN2). We developed a novel multiplexed SRM assay using LC-MS/MS to measure five proteins related to GDF8 and GDF11 signaling, and in addition, eotaxin, and oxytocin. Eighteen peptides consisting of 54 transitions were monitored and validated in pooled human plasma. In 24 adults, the mean (SD) concentrations (ng/mL) were as follows: GDF8 propeptide, 11.0 (2.4); GDF8 mature protein, 25.7 (8.0); GDF11 propeptide, 21.3 (10.9); GDF11 mature protein, 16.5 (12.4); FST, 29.8 (7.1); FST cleavage form FST303, 96.4 (69.2); WFIKKN1, 38.3 (8.3); WFIKKN2, 32.2 (10.5); oxytocin, 1.9 (0.9); and eotaxin, 2.3 (0.5). This novel multiplexed SRM assay should facilitate the study of the relationships of these proteoforms with major aging phenotypes.

Indexed as

AgingBiomarkersBone Morphogenetic ProteinsCarrier ProteinsChemokine CCL11FemaleGrowth Differentiation FactorsHumansIntercellular Signaling Peptides and ProteinsMaleMiddle AgedMyostatinOxytocinPhenotypeProtein IsoformsProteinsBiomarkersBone Morphogenetic ProteinsCarrier ProteinsChemokine CCL11GDF11 protein, humanGrowth Differentiation FactorsIntercellular Signaling Peptides and ProteinsMyostatinOxytocinProtein IsoformsProteinsProteomeWFIKKN1 protein, humanWFIKKN2 protein, humanAgingEotaxinFollistatinGrowth/differentiation factor 11Growth/differentiation factor 8OxytocinWFIKKN1WFIKKN2

Identifiers

PMID28508553
PMCPMC5863538
OpenAlexW2614252892

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.