ArticleVeterinary world2017
Pathotypic characterization of Newcastle disease virus isolated from vaccinated chicken in West Java, Indonesia.
Article in Veterinary world, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- First report of molecular genotyping, pathotyping, and histopathological characterization of lentogenic genotype II Newcastle disease virus circulating in young ostrich flocks in Egypt.Veterinary world · 2026Article
- Gamma-irradiated Newcastle disease virus: an alternative inactivated oncolytic virotherapy.Immunology and cell biology · 2026Article
- Epidemiological distribution of respiratory viral pathogens in marketable vaccinated broiler chickens in five governorates in the Nile Delta, Egypt, from January 2022 to October 2022.Veterinary world · 2024Article
- Newcastle disease virus: the past and current situation in Indonesia.Journal of veterinary science · 2024Review
- Production of hyperimmune serum against genotype VII Newcastle disease virus in rabbits with several applications.Journal of advanced veterinary and animal research · 2022Article
- Genetic characterization and distribution of the virus in chicken embryo tissues infected with Newcastle disease virus isolated from commercial and native chickens in Indonesia.Veterinary world · 2022Article
- Phylogenetic studies of Newcastle disease virus isolated from poultry flocks in South Sulawesi Province, Indonesia, in 2019.Journal of advanced veterinary and animal research · 2021Article
- Phylogenetic relationship and genotype variation of six Newcastle disease viruses isolated from duck in Indonesia.Veterinary world · 2021Article
- Development of a coagglutination kit as a rapid test for diagnosing Newcastle disease in poultry.Veterinary world · 2020Article
- The efficacy of binary ethylenimine-inactivated vaccines of Gianyar-1/AK/2014 virulent strain in protecting chickens against Tabanan-1/ARP/2017 virulent Newcastle disease virus isolates.Veterinary world · 2019Article
- Production and characterization of Newcastle disease antibody as a reagent to develop a rapid immunodiagnostic test tool.Veterinary world · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimThis research was conducted to differentiate and characterize eight Newcastle disease virus (NDV) isolates collected from vaccinated chicken at commercial flocks in West Java, Indonesia, in 2011, 2014 and 2015 by pathotype specific primers. MATERIALS AND
methodsA total of eight NDV isolates collected from clinical outbreaks among commercial vaccinated flocks in West Java, Indonesia, in 2011, 2014, and 2015 were used in this study. Reverse transcription-polymerase chain reaction was used to detect and differentiate virulence of NDV strains, using three sets of primers targeting their M and F gene. First primers were universal primers to detect NDV targeting matrix (M) gene. Other two sets of primers were specific for the fusion (F) gene cleavage site sequence of virulent and avirulent NDV strains.
resultsOur results showed that three isolates belong to NDV virulent strains, and other five isolates belong to NDV avirulent strains. The nucleotide sequence of the F protein cleavage site showed
conclusionResult from the current study suggested that NDV virulent strain were circulating among vaccinated chickens in West Java, Indonesia; this might possess a risk of causing ND outbreaks and causing economic losses within the poultry industry.
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