ArticleProceedings of the National Academy of Sciences of the United States of America2017
Quantitative assessment of passive electrical properties of the cardiac T-tubular system by FRAP microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- A new approach to the determination of tubular membrane capacitance: passive membrane electrical properties under reduced electrical conductivity of the extracellular solution.Pflugers Archiv : European journal of physiology · 2022Article
- Optogenetic manipulation of cardiac electrical dynamics using sub-threshold illumination: dissecting the role of cardiac alternans in terminating rapid rhythms.Basic research in cardiology · 2022Article
- Fraction of the T-Tubular Membrane as an Important Parameter in Cardiac Cellular Electrophysiology: A New Way of Estimation.Frontiers in physiology · 2022Article
- Review
- T-tubule remodeling in human hypertrophic cardiomyopathy.Journal of muscle research and cell motility · 2021Review
- Beat-by-Beat Cardiomyocyte T-Tubule Deformation Drives Tubular Content Exchange.Circulation research · 2021Article
- Novel Optics-Based Approaches for Cardiac Electrophysiology: A Review.Frontiers in physiology · 2021Review
- The Physiology and Pathophysiology of T-Tubules in the Heart.Frontiers in physiology · 2021Review
- The control mechanisms of heart rate dynamics in a new heart rate nonlinear time series model.Scientific reports · 2020Article
- Influence of the tubular network on the characteristics of calcium transients in cardiac myocytes.PloS one · 2020Article
- Modeling Depolarization Delay, Sodium Currents, and Electrical Potentials in Cardiac Transverse Tubules.Frontiers in physiology · 2019Article
- Species differences in the morphology of transverse tubule openings in cardiomyocytes.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2018Article
- Real-time optical manipulation of cardiac conduction in intact hearts.The Journal of physiology · 2018Article
- Solute movement in the t-tubule system of rabbit and mouse cardiomyocytes.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Uncovering an electrically heterogeneous cardiomyocyte by FRAP-quantified diffusion in the T-tubules.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Reply to Entcheva: The impact of T-tubules on action potential propagation in cardiac tissue.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Diffusional and Electrical Properties of T-Tubules Are Governed by Their Constrictions and Dilations.Biophysical journal · 2018Article
- Axial Tubule Junctions Activate Atrial CaFrontiers in physiology · 2018Article
- Interplay Between Sub-Cellular Alterations of Calcium Release and T-Tubular Defects in Cardiac Diseases.Frontiers in physiology · 2018Article
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13 authors at 3 institutions in 3 countries.
Funding
Abstract
Well-coordinated activation of all cardiomyocytes must occur on every heartbeat. At the cell level, a complex network of sarcolemmal invaginations, called the transverse-axial tubular system (TATS), propagates membrane potential changes to the cell core, ensuring synchronous and uniform excitation-contraction coupling. Although myocardial conduction of excitation has been widely described, the electrical properties of the TATS remain mostly unknown. Here, we exploit the formal analogy between diffusion and electrical conductivity to link the latter with the diffusional properties of TATS. Fluorescence recovery after photobleaching (FRAP) microscopy is used to probe the diffusion properties of TATS in isolated rat cardiomyocytes: A fluorescent dextran inside TATS lumen is photobleached, and signal recovery by diffusion of unbleached dextran from the extracellular space is monitored. We designed a mathematical model to correlate the time constant of fluorescence recovery with the apparent diffusion coefficient of the fluorescent molecules. Then, apparent diffusion is linked to electrical conductivity and used to evaluate the efficiency of the passive spread of membrane depolarization along TATS. The method is first validated in cells where most TATS elements are acutely detached by osmotic shock and then applied to probe TATS electrical conductivity in failing heart cells. We find that acute and pathological tubular remodeling significantly affect TATS electrical conductivity. This may explain the occurrence of defects in action potential propagation at the level of single T-tubules, recently observed in diseased cardiomyocytes.
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