Evidence map›Paper›PMID 28506465›Full record

ArticleCell stem cell2017

Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution.

Russell B Fletcher, Diya Das, Levi Gadye, Kelly N Street, Ariane Baudhuin, Allon Wagner, Michael B Cole, Quetzal Flores, Yoon Gi Choi, Nir Yosef and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell stem cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 145 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
145citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

145 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  8. Review
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  11. Novel mechanisms of chemosensory adaptation to the cave environment.bioRxiv : the preprint server for biology · 2026
    Article
  12. Article
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  14. Article
  15. Review
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  17. Review
  18. Olfactory models as a platform for neuroscience research.Frontiers in cellular neuroscience · 2026
    Review
  19. Review
  20. Article

85 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Russell B FletcherDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Diya DasDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Levi GadyeHelen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, USA.
Kelly N StreetDivision of Biostatistics, University of California, Berkeley, CA 94720, USA; Center for Computational Biology, University of California, Berkeley, CA 94720, USA.
Ariane BaudhuinDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Allon WagnerDepartment of Electrical Engineering and Computer Science, University of California, Berkeley, CA 94720, USA; Center for Computational Biology, University of California, Berkeley, CA 94720, USA.
Michael B ColeDepartment of Physics, University of California, Berkeley, CA 94720, USA; Center for Computational Biology, University of California, Berkeley, CA 94720, USA.
Quetzal FloresDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Yoon Gi ChoiQB3 Functional Genomics Laboratory, University of California, Berkeley, CA 94720, USA.
Nir YosefDepartment of Electrical Engineering and Computer Science, University of California, Berkeley, CA 94720, USA; Center for Computational Biology, University of California, Berkeley, CA 94720, USA.
Elizabeth PurdomDepartment of Statistics, University of California, Berkeley, CA 94720, USA; Center for Computational Biology, University of California, Berkeley, CA 94720, USA.
Sandrine DudoitDivision of Biostatistics, University of California, Berkeley, CA 94720, USA; Department of Statistics, University of California, Berkeley, CA 94720, USA; Center for Computational Biology, University of California, Berkeley, CA 94720, USA.
Davide RissoDivision of Biostatistics, University of California, Berkeley, CA 94720, USA.
John NgaiDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA; Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, USA; QB3 Functional Genomics Laboratory, University of California, Berkeley, CA 94720, USA. Electronic address: jngai@berkeley.edu.

Funding

GENOMICST32HG000047 · NHGRI · UNIVERSITY OF CALIFORNIA BERKELEY · PI RASMUS NIELSEN, Daniel Soleyman Rokhsar · 2000 to 2026
$13.5M
Supplement for Programs of Gene Expression in Olfactory NeurogenesisR01DC007235 · NIDCD · UNIVERSITY OF CALIFORNIA BERKELEY · PI DUDOIT, SANDRINE · 2005 to 2022
$6.6M
Classification of Cortical Neurons by Single Cell TranscriptomicsU01MH105979 · NIMH · UNIVERSITY OF CALIFORNIA BERKELEY · PI NGAI, JOHN J. · 2014 to 2016
$4.5M
Stem Cell Engineering Training ProgramT32GM098218 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI HEALY, KEVIN EDWARD, SCHAFFER, DAVID V · 2011 to 2020
$1.8M
The UC Berkeley MARC: Leadership and Innovation in the Biological Sciences.T34GM092702 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI MATSUI, JOHN · 2010 to 2014
$1.7M
DEVELOPMENT OF GENOMIC INSTRUMENTATIONK22HG000047 · NHGRI · UNIVERSITY OF CALIFORNIA IRVINE · PI BRODY, JAMES P · 2000 to 2004
$964k
Genome Sequencer FLXS10RR029668 · NCRR · UNIVERSITY OF CALIFORNIA BERKELEY · PI NGAI, JOHN J. · 2010 to 2010
$623k
Age-related changes in the neural stem cells of the olfactory epitheliumK01AG045344 · NIA · UNIVERSITY OF CALIFORNIA BERKELEY · PI FLETCHER, RUSSELL B · 2014 to 2016
$337k
NCRR NIH HHS S10 RR029668NHGRI NIH HHS K22 HG000047NHGRI NIH HHS T32 HG000047NIA NIH HHS K01 AG045344NIDCD NIH HHS R01 DC007235NIGMS NIH HHS T32 GM098218NIGMS NIH HHS T34 GM092702NIMH NIH HHS U01 MH105979
6 · The paper itself

Abstract

A detailed understanding of the paths that stem cells traverse to generate mature progeny is vital for elucidating the mechanisms governing cell fate decisions and tissue homeostasis. Adult stem cells maintain and regenerate multiple mature cell lineages in the olfactory epithelium. Here we integrate single-cell RNA sequencing and robust statistical analyses with in vivo lineage tracing to define a detailed map of the postnatal olfactory epithelium, revealing cell fate potentials and branchpoints in olfactory stem cell lineage trajectories. Olfactory stem cells produce support cells via direct fate conversion in the absence of cell division, and their multipotency at the population level reflects collective unipotent cell fate decisions by single stem cells. We further demonstrate that Wnt signaling regulates stem cell fate by promoting neuronal fate choices. This integrated approach reveals the mechanisms guiding olfactory lineage trajectories and provides a model for deconstructing similar hierarchies in other stem cell niches.

Indexed as

Adult Stem CellsMultipotent Stem CellsOlfactory MucosaAnimalsCell DivisionMiceMice, TransgenicWnt Signaling Pathwaybranching lineageHBChorizontal basal celllineage trajectoryolfactory epitheliumscRNA-seqstem cellsWnt

Identifiers

PMID28506465
PMCPMC5484588

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.