ArticleNature neuroscience2017
A fluoro-Nissl dye identifies pericytes as distinct vascular mural cells during in vivo brain imaging.
Article in Nature neuroscience, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
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Who cites it
75 citing papers in PubMed, 111 citations in OpenAlex.
- Transient weakening of the blood-brain barrier in neonatal mice.Fluids and barriers of the CNS · 2026Article
- Programming Brain Cell-Type-Selective Delivery In Vivo with Transporter-Guided Therapeutics.bioRxiv : the preprint server for biology · 2026Article
- Atto 643 Carboxy Selectively Labels Astrocytes with Minimal Oligodendrocyte Cross-Reactivity.bioRxiv : the preprint server for biology · 2026Article
- Persistent dysmorphology and dysfunction of the brain microvasculature following repeated trauma.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2026Article
- Eppur si muove: the dynamic brain pericyte.Fluids and barriers of the CNS · 2025Review
- Pericytes in the optic nerve head.Progress in retinal and eye research · 2025Review
- Impact of NA-1 on Pericyte-Driven Vasoconstriction and Its Role in No-Reflow During Cerebral Ischemia-Reperfusion.CNS neuroscience & therapeutics · 2025Article
- Dynamic changes in the structure and function of brain mural cells around chronically implanted microelectrodes.Biomaterials · 2025Article
- Expression of alpha smooth muscle actin decreases with ageing and increases upon lumen obstruction in mouse brain pericytes.GeroScience · 2025Article
- Shallow-angle intracranial cannula for repeated infusion andNeurophotonics · 2025Article
- Neurovascular coupling: a review of spontaneous neocortical dynamics linking neuronal activity to hemodynamics and what we have learned from the rodent brain.Journal of neurophysiology · 2025Review
- Shallow-angle intracranial cannula for repeated infusion and in vivo imaging with multiphoton microscopy.bioRxiv : the preprint server for biology · 2025Article
- KProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Inhibition of Angiopoietin-2 rescues sporadic brain arteriovenous malformations by reducing pericyte loss.Angiogenesis · 2024Article
- mTORC1 Signaling in Brain Endothelial Progenitors Contributes to CCM Pathogenesis.Circulation research · 2024Article
- Distinct potassium channel types in brain capillary pericytes.Biophysical journal · 2024Article
- The role of cardiac pericytes in health and disease: therapeutic targets for myocardial infarction.Nature reviews. Cardiology · 2024Review
- Imaging the Retinal Vascular Mural Cells In Vivo: Elucidating the Timeline of Their Loss in Diabetic Retinopathy.Arteriosclerosis, thrombosis, and vascular biology · 2024Article
- Targeting Pericytes for Functional Recovery in Ischemic Stroke.Neuromolecular medicine · 2023Review
- Amyloid beta accumulation in TgF344-AD rats is associated with reduced cerebral capillary endothelial Kir2.1 expression and neurovascular uncoupling.GeroScience · 2023Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Pericytes and smooth muscle cells are integral components of the brain microvasculature. However, no techniques exist to unambiguously identify these cell types, greatly limiting their investigation in vivo. Here we show that the fluorescent Nissl dye NeuroTrace 500/525 labels brain pericytes with specificity, allowing high-resolution optical imaging in the live mouse. We demonstrate that capillary pericytes are a population of mural cells with distinct morphological, molecular and functional features that do not overlap with precapillary or arteriolar smooth muscle actin-expressing cells. The remarkable specificity for dye uptake suggests that pericytes have molecular transport mechanisms not present in other brain cells. We demonstrate feasibility of longitudinal pericyte imaging during microvascular development and aging and in models of brain ischemia and Alzheimer's disease. The ability to easily label pericytes in any mouse model opens the possibility of a broad range of investigations of mural cells in vascular development, neurovascular coupling and neuropathology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.