ArticleNature biotechnology2017
Rapid cloning of genes in hexaploid wheat using cultivar-specific long-range chromosome assembly.
Article in Nature biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers.
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Who cites it
111 citing papers in PubMed.
- Potential unlocked: an atlas of cloned wheat genes for genome engineering and breeding.The New phytologist · 2026Review
- Dissecting adult plant resistance to stripe and leaf rust in advanced Indian wheat breeding lines through a genome-wide association study.BMC plant biology · 2026Article
- The pan-NLRome and diversity of Watkins wheat provide genetic resources for improving disease resistance and adaptation.Plant communications · 2025Article
- Genome-assisted identification of wheat leaf rust resistance gene Lr.ace-4A/Lr30.Nature communications · 2025Article
- Discovery of functional NLRs using expression level, high-throughput transformation and large-scale phenotyping.Nature plants · 2025Article
- An optimized disease resistance gene cloning workflow for wheat.Nature communications · 2025Article
- Identification of leaf rust resistance loci in hard winter wheat using genome-wide association mapping.The plant genome · 2025Article
- Counterintuitive DNA destabilization by monovalent salt at high concentrations due to overcharging.Nature communications · 2025Article
- k-mer genome-wide association study for anthracnose and BCMV resistance in a Phaseolus vulgaris Andean Diversity Panel.The plant genome · 2024Article
- A single NLR gene confers resistance to leaf and stripe rust in wheat.Nature communications · 2024Article
- Sexy ways: approaches to studying plant sex chromosomes.Journal of experimental botany · 2024Review
- Fine-mapping of LrN3B on wheat chromosome arm 3BS, one of the two complementary genes for adult-plant leaf rust resistance.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Plant pangenomes for crop improvement, biodiversity and evolution.Nature reviews. Genetics · 2024Review
- Genome-wide atlas of rust resistance loci in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Genome wide association study of seedling and adult plant leaf rust resistance in two subsets of barley genetic resources.Scientific reports · 2024Article
- Genome-wide association analysis uncovers rice blast resistance alleles of Ptr and Pia.Communications biology · 2024Article
- Barley MLA3 recognizes the host-specificity effector Pwl2 from Magnaporthe oryzae.The Plant cell · 2024Article
- Phenotyping and Exploitation of Kompetitive Allele-Specific PCR Assays for Genes Underpinning Leaf Rust Resistance in New Spring Wheat Mutant Lines.Current issues in molecular biology · 2024Article
- Dissection of a rapidly evolving wheat resistance gene cluster by long-read genome sequencing accelerated the cloning of Pm69.Plant communications · 2024Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cereal crops such as wheat and maize have large repeat-rich genomes that make cloning of individual genes challenging. Moreover, gene order and gene sequences often differ substantially between cultivars of the same crop species. A major bottleneck for gene cloning in cereals is the generation of high-quality sequence information from a cultivar of interest. In order to accelerate gene cloning from any cropping line, we report 'targeted chromosome-based cloning via long-range assembly' (TACCA). TACCA combines lossless genome-complexity reduction via chromosome flow sorting with Chicago long-range linkage to assemble complex genomes. We applied TACCA to produce a high-quality (N50 of 9.76 Mb) de novo chromosome assembly of the wheat line CH Campala Lr22a in only 4 months. Using this assembly we cloned the broad-spectrum Lr22a leaf-rust resistance gene, using molecular marker information and ethyl methanesulfonate (EMS) mutants, and found that Lr22a encodes an intracellular immune receptor homologous to the Arabidopsis thaliana RPM1 protein.
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