ArticleThe Histochemical journal1988
A quantitative histochemical study of 5'-nucleotidase activity in rat liver using the lead salt method and polyvinyl alcohol.
Article in The Histochemical journal, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- CD73 (ecto-5'-nucleotidase) hepatocyte levels differ across mouse strains and contribute to mallory-denk body formation.Hepatology (Baltimore, Md.) · 2013Article
- SP1-dependent induction of CD39 facilitates hepatic ischemic preconditioning.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Effects of ischaemia and reperfusion on NADH coenzyme Q reductase activity in rat liver.The Histochemical journal · 1999Article
- Demonstration of 5'-nucleotidase activity in unfixed cryostat sections of rat liver using a combined light- and electron-microscope procedure.The Histochemical journal · 1995Article
- Distribution of ecto-5'-nucleotidase in the rat liver: effect of anaemia.Histochemistry · 1994Article
- The initial reaction velocities of lactate dehydrogenase in various cell types.The Histochemical journal · 1994Article
- Quantitative aspects of enzyme histochemistry on sections of freeze-substituted glycol methacrylate-embedded rat liver.Histochemistry · 1993Article
- In situ kinetic measurements of D-amino acid oxidase in rat liver with respect to its substrate specificity.The Histochemical journal · 1993Article
- Kinetic analysis of lactate dehydrogenase in situ in mouse liver determined with a quantitative histochemical technique.The Histochemical journal · 1993Article
- The effects of storage on the retention of enzyme activity in cryostat sections. A quantitative histochemical study on rat liver.The Histochemical journal · 1993Article
- Pitfalls in the light microscopical detection of NADH oxidase.The Histochemical journal · 1990Article
- Polyvinyl alcohol and other tissue protectants in enzyme histochemistry: a consumer's guide.The Histochemical journal · 1989Review
- Histochemical detection of glycogen phosphorylase activity as parameter for early ischemic damage in rat heart.Basic research in cardiologyArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
5'-Nucleotidase (EC 3.1.3.5) activity was demonstrated in cryostat sections of rat liver using the Wachstein-Meisel medium and polyvinyl alcohol as tissue stabilizer. Optimum activity was obtained using an incubation medium containing 5 mM AMP, 10 mM magnesium chloride, 7.2 mM lead nitrate, 0.1 M Tris-maleate buffer, pH 7.2, and 17% (w/v) polyvinyl alcohol (Sigma, type III). The activity was localized at the bile canalicular and sinusoidal side of the plasma membranes of liver parenchymal cells as well as in the plasma membranes of endothelial cells of central veins and in fibroblasts surrounding portal tracts. The reaction was specific for 5'-nucleotidase because it was inhibited by ADP. Alkaline phosphatase did not interfere in the reaction. Cytophotometric analysis revealed a linear relationship between the formation of the final reaction product and incubation times up to 20 min and section thicknesses up to 8 micron. The activity in pericentral zones was 1.35 times the activity in periportal zones. The Michaelis constant for AMP was 1.4 mM in pericentral zones and 0.8 mM in periportal zones, suggesting that the bile canalicular and sinusoidal enzymes differ in their kinetic characteristics.
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