Evidence map›Paper›PMID 28500755›Full record

ArticleeLife2017

Enhanced FIB-SEM systems for large-volume 3D imaging.

C Shan Xu, Kenneth J Hayworth, Zhiyuan Lu, Patricia Grob, Ahmed M Hassan, José G García-Cerdán, Krishna K Niyogi, Eva Nogales, Richard J Weinberg, Harald F Hess

Abstract read
In one paragraph

Article in eLife, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 198 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
198citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

198 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Perfusion fixation in brain banking: a systematic review.Acta neuropathologica communications · 2019
    Pooled it
  3. Article
  4. Dystrophic changes of nigrostriatal axons harboring aProceedings of the National Academy of Sciences of the United States of America · 2026
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138 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

C Shan XuJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.ORCID 0000-0002-8564-7836
Kenneth J HayworthJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
Zhiyuan LuJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
Patricia GrobHoward Hughes Medical Institute, Molecular and Cell Biology Department, University of California, Berkeley, United States.
Ahmed M HassanHoward Hughes Medical Institute, Molecular and Cell Biology Department, University of California, Berkeley, United States.
José G García-CerdánHoward Hughes Medical Institute, Plant and Microbial Biology Department, University of California, Berkeley, United States.
Krishna K NiyogiHoward Hughes Medical Institute, Plant and Microbial Biology Department, University of California, Berkeley, United States.
Eva NogalesHoward Hughes Medical Institute, Molecular and Cell Biology Department, University of California, Berkeley, United States.ORCID 0000-0001-9816-3681
Richard J WeinbergDepartment of Cell Biology and Physiology, University of North Carolina, North Carolina, United States.
Harald F HessJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.

Funding

The Supramolecular Architecture of the Postsynaptic DensityR01NS039444 · NINDS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI WEINBERG, RICHARD J · 2000 to 2021
$6.1M
NINDS NIH HHS R01 NS039444
6 · The paper itself

Abstract

Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) can automatically generate 3D images with superior z-axis resolution, yielding data that needs minimal image registration and related post-processing. Obstacles blocking wider adoption of FIB-SEM include slow imaging speed and lack of long-term system stability, which caps the maximum possible acquisition volume. Here, we present techniques that accelerate image acquisition while greatly improving FIB-SEM reliability, allowing the system to operate for months and generating continuously imaged volumes > 10

Indexed as

AnimalsBrainChlamydomonas reinhardtiiDrosophilaImaging, Three-DimensionalMice, Inbred C57BLMicroscopy, Electron, ScanningNeurons3D cellular structurecell biologyconnectomicselectron microscopyFIBimagingneuroscience

Identifiers

PMID28500755
PMCPMC5476429

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.