ArticleScientific reports2017
Microgravity induces inhibition of osteoblastic differentiation and mineralization through abrogating primary cilia.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 58 citations in OpenAlex.
- Primary cilia: master conductors of cellular communication in development and disease.Nature reviews. Nephrology · 2026Review
- Impact of Microgravity on Cytoskeletal Dynamics, Protein Transport, and Signaling Networks: Potential Therapeutic Opportunities for Skin Health.International journal of molecular sciences · 2026Review
- Effects of microgravity mechanotransduction in bone tissue and cells: systematic review on primary cilium-dependent mechanisms.NPJ microgravity · 2026Article
- The hierarchical stratum response system of organism to microgravity during spaceflight.Military Medical Research · 2026Review
- Ciliary-Mediated Mechanotransduction in Skeletal Development and Diseases.Results and problems in cell differentiation · 2026Review
- Enhanced Ciliogenesis of Human Bronchial Epithelial Cells by Simulated Microgravity.Life (Basel, Switzerland) · 2025Article
- Focal Adhesion Kinase Alleviates Simulated Microgravity-Induced Inhibition of Osteoblast Differentiation by Activating Transcriptional Wnt/β-Catenin-BMP2-COL1 and Metabolic SIRT1-PGC-1α-CPT1A Pathways.International journal of molecular sciences · 2025Article
- Melatonin Regulates Osteoblast Differentiation through the m6A Reader hnRNPA2B1 under Simulated Microgravity.Current issues in molecular biology · 2024Article
- Blood-brain-barrier modeling with tissue chips for research applications in space and on Earth.Frontiers in space technologies · 2023Article
- Maternal protein deficiency alters primary cilia length in renal tubular and impairs kidney development in fetal rat.Frontiers in nutrition · 2023Article
- Protection of primary cilia is an effective countermeasure against the impairment of osteoblast function induced by simulated microgravity.Journal of cellular and molecular medicine · 2023Article
- Impairment of 7F2 osteoblast function by simulated partial gravity in a Random Positioning Machine.NPJ microgravity · 2022Article
- Activation of Focal Adhesion Kinase Restores Simulated Microgravity-Induced Inhibition of Osteoblast Differentiation via Wnt/Β-Catenin Pathway.International journal of molecular sciences · 2022Article
- Exposure to Random Positioning Machine Alters the Mineralization Process and PTX3 Expression in the SAOS-2 Cell Line.Life (Basel, Switzerland) · 2022Article
- Mechanical regulation of bone remodeling.Bone research · 2022Review
- Primary cilia: The central role in the electromagnetic field induced bone healing.Frontiers in pharmacology · 2022Review
- Large-scale analysis reveals spatiotemporal circadian patterns of cilia transcriptomes in the primate brain.Journal of neuroscience research · 2021Article
- The effects of locomotion on bone marrow mesenchymal stem cell fate: insight into mechanical regulation and bone formation.Cell & bioscience · 2021Review
- Immunity in Space: Prokaryote Adaptations and Immune Response in Microgravity.Life (Basel, Switzerland) · 2021Review
- Antioxidant Strategy to Prevent Simulated Microgravity-Induced Effects on Bone Osteoblasts.International journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is well documented that microgravity in space environment leads to bone loss in astronauts. These physiological changes have also been validated by human and animal studies and modeled in cell-based analogs. However, the underlying mechanisms are elusive. In the current study, we identified a novel phenomenon that primary cilia (key sensors and functioning organelles) of rat calvarial osteoblasts (ROBs) gradually shrank and disappeared almost completely after exposure to simulated microgravity generated by a random positioning machine (RPM). Along with the abrogation of primary cilia, the differentiation, maturation and mineralization of ROBs were inhibited. We also found that the disappearance of primary cilia was prevented by treating ROBs with cytochalasin D, but not with LiCl or dynein light chain Tctex-type 1 (Dynlt1) siRNA. The repression of the differentiation, maturation and mineralization of ROBs was effectively offset by cytochalasin D treatment in microgravity conditions. Blocking ciliogenesis using intraflagellar transport protein 88 (IFT88) siRNA knockdown inhibited the ability of cytochalasin D to counteract this reduction of osteogenesis. These results indicate that the abrogation of primary cilia may be responsible for the microgravity's inhibition on osteogenesis. Reconstruction of primary cilia may become a potential strategy against bone loss induced by microgravity.
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