Evidence map›Paper›PMID 28499842›Full record

ArticleJournal of neuroscience methods2017

Establishing the ferret as a gyrencephalic animal model of traumatic brain injury: Optimization of controlled cortical impact procedures.

Susan C Schwerin, Elizabeth B Hutchinson, Kryslaine L Radomski, Kapinga P Ngalula, Carlo M Pierpaoli, Sharon L Juliano

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroscience methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 40 citations in OpenAlex.

  1. Review
  2. Standardizing the lateral fluid percussion model of trauma in marmosets (Callithrix jacchus): regional vulnerability and inflammatory response.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas · 2026
    Article
  3. Review
  4. Article
  5. Metabolic Imaging of Hyperpolarized [1-International journal of molecular sciences · 2025
    Article
  6. Article
  7. Article
  8. Development of multisensory processing in ferret parietal cortex.The European journal of neuroscience · 2023
    Article
  9. Article
  10. Review
  11. Review
  12. Article
  13. Expression of GFAP and Tau Following Blast Exposure in the Cerebral Cortex of Ferrets.Journal of neuropathology and experimental neurology · 2021
    Article
  14. Article
  15. The Ferret as a Model System for Neocortex Development and Evolution.Frontiers in cell and developmental biology · 2021
    Review
  16. Review
  17. Review
  18. Article
  19. Biomechanical simulation of traumatic brain injury in the rat.Clinical biomechanics (Bristol, Avon) · 2019
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Susan C SchwerinDepartment of Anatomy Physiology and Genetics, Uniformed Services University of Health Sciences, 4301 Jones Bridge Rd, Building B, Bethesda, MD, 20814, USA. Electronic address: susan.schwerin.ctr@usuhs.edu.
Elizabeth B HutchinsonNational Institute of Child Health and Development, National Institutes of Health, 13 South Drive, Building 13, Bethesda, MD, 20892, USA. Electronic address: elizabeth.hutchinson@nih.gov.
Kryslaine L RadomskiDepartment of Anatomy Physiology and Genetics, Uniformed Services University of Health Sciences, 4301 Jones Bridge Rd, Building B, Bethesda, MD, 20814, USA. Electronic address: kryslaine.radomski.ctr@usuhs.edu.
Kapinga P NgalulaDepartment of Anatomy Physiology and Genetics, Uniformed Services University of Health Sciences, 4301 Jones Bridge Rd, Building B, Bethesda, MD, 20814, USA. Electronic address: kabaselepatricia@gmail.com.
Carlo M PierpaoliNational Institute of Child Health and Development, National Institutes of Health, 13 South Drive, Building 13, Bethesda, MD, 20892, USA. Electronic address: cp1a@nih.gov.
Sharon L JulianoDepartment of Anatomy Physiology and Genetics, Uniformed Services University of Health Sciences, 4301 Jones Bridge Rd, Building B, Bethesda, MD, 20814, USA; Program in Neuroscience, Uniformed Services University of Health Sciences, 4301 Jones Bridge Rd, Building B, Bethesda, MD, 20814, USA. Electronic address: sharon.juliano@usuhs.edu.
Uniformed Services University of the Health Sciences · USNational Institutes of Health · US

Funding

Quantitative Imaging in experimental models of traumatic brain injuryZIAEB000089 · NIBIB · NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING · PI PIERPAOLI, CARLO · 2017 to 2020
$1.7M
Intramural NIH HHS Z99 EB999999
6 · The paper itself

Abstract

backgroundAlthough rodent TBI studies provide valuable information regarding the effects of injury and recovery, an animal model with neuroanatomical characteristics closer to humans may provide a more meaningful basis for clinical translation. The ferret has a high white/gray matter ratio, gyrencephalic neocortex, and ventral hippocampal location. Furthermore, ferrets are amenable to behavioral training, have a body size compatible with pre-clinical MRI, and are cost-effective. NEW

methodsWe optimized the surgical procedure for controlled cortical impact (CCI) using 9 adult male ferrets. We used subject-specific brain/skull morphometric data from anatomical MRIs to overcome across-subject variability for lesion placement. We also reflected the temporalis muscle, closed the craniotomy, and used antibiotics. We then gathered MRI, behavioral, and immunohistochemical data from 6 additional animals using the optimized surgical protocol: 1 control, 3 mild, and 1 severely injured animals (surviving one week) and 1 moderately injured animal surviving sixteen weeks.

resultsThe optimized surgical protocol resulted in consistent injury placement. Astrocytic reactivity increased with injury severity showing progressively greater numbers of astrocytes within the white matter. The density and morphological changes of microglia amplified with injury severity or time after injury. Motor and cognitive impairments scaled with injury severity. COMPARISON WITH EXISTING METHOD(S): The optimized surgical methods differ from those used in the rodent, and are integral to success using a ferret model.

conclusionsWe optimized ferret CCI surgery for consistent injury placement. The ferret is an excellent animal model to investigate pathophysiological and behavioral changes associated with TBI.

Indexed as

Disease Models, AnimalAnimalsBrain Injuries, TraumaticBrain MappingCalcium-Binding ProteinsCraniotomyDNA-Binding ProteinsExploratory BehaviorFerretsGaitGlial Fibrillary Acidic ProteinImage Processing, Computer-AssistedMagnetic Resonance ImagingMaleMicrofilament ProteinsNeocortexAIF1 protein, humanCalcium-Binding ProteinsDNA-Binding ProteinsGlial Fibrillary Acidic ProteinMicrofilament ProteinsAcuteAntibioticsAstrocytesBehaviorCCIChronicCognitiveCraniotomyImmunohistochemistryImpairmentInflammationLandmarksMicrogliaMotorMRIReproducibilitySurgeryTBITemporalisWhite matter

Identifiers

PMID28499842
PMCPMC6320441
OpenAlexW2613296465

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.