ArticleJournal of translational medicine2017
Bovis Bacillus Calmette-Guerin (BCG) infection induces exosomal miRNA release by human macrophages.
Article in Journal of translational medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Impact of interspecies colostrum and milk replacement on circulating sncRNA dynamics of neonatal goat kids.RNA biology · 2026Article
- Role of Exosomes During Mycobacterium Tuberculosis Infection: A Double-Edged Sword.Biomedicines · 2026Review
- Extracellular Vesicles of Mycobacterium tuberculosis Serve as a Virulence Factor - Current Research Applications.Current microbiology · 2026Review
- Downregulated Plasma-Derived Extracellular Vesicle MicroRNAs as Novel Biomarkers for Enhanced Tuberculosis Diagnosis.Journal of immunology research · 2026Article
- Exploring the Potential of Exosomes as Biomarkers in Tuberculosis and Other Diseases.International journal of molecular sciences · 2024Review
- Mmu-let-7a-5p inhibits macrophage apoptosis by targeting CASP3 to increase bacterial load and facilities mycobacterium survival.PloS one · 2024Article
- Significance of extracellular vesicles in orchestration of immune responses inFrontiers in cellular and infection microbiology · 2024Review
- Current landscape of exosomes in tuberculosis development, diagnosis, and treatment applications.Frontiers in immunology · 2024Review
- microRNA hsa-miR-425-5p and hsa-miR-4523 expressions as biomarkers of active pulmonary tuberculosis, latent tuberculosis infection, and lymph node tuberculosis.Non-coding RNA research · 2023Article
- Mmu-miR-25-3p promotes macrophage autophagy by targeting DUSP10 to reduce mycobacteria survival.Frontiers in cellular and infection microbiology · 2023Article
- The diagnosis of tuberculous meningitis: advancements in new technologies and machine learning algorithms.Frontiers in microbiology · 2023Review
- Differential expression analysis of miRNAs in macrophage-derived exosomes in the tuberculosis-infected bone microenvironment.Frontiers in microbiology · 2023Article
- Editorial: Exosomes and exosomal miRNAs as biomarkers in infection with Mycobacterium tuberculosis.Frontiers in cellular and infection microbiology · 2023Article
- Functions of exosomal non-coding RNAs to the infection withFrontiers in immunology · 2023Review
- Small RNA sequencing and bioinformatics analysis of RAW264.7-derived exosomes after Mycobacterium Bovis Bacillus Calmette-Guérin infection.BMC genomics · 2022Article
- Understanding microRNAs in the Context of Infection to Find New Treatments against Human Bacterial Pathogens.Antibiotics (Basel, Switzerland) · 2022Review
- Extracellular vesicles from serum samples of mycobacteria patients induced cell death of THP-1 monocyte and PBMC.BMC pulmonary medicine · 2022Article
- Extracellular Vesicles: Recent Insights Into the Interaction Between Host and Pathogenic Bacteria.Frontiers in immunology · 2022Review
- The Role of Extracellular Vesicles from Human Macrophages on Host-Pathogen Interaction.International journal of molecular sciences · 2021Review
- Regulatory roles of extracellular vesicles in immune responses againstWorld journal of clinical cases · 2021Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
backgroundTuberculosis (TB) remains a significant global health concern and its diagnosis is challenging due to the limitations in the specificity and sensitivity of the current diagnostic tests. Exosomes are bioactive 30-100 nm vesicles produced by most cell types and are found in almost all human body fluids. Exosomal microRNAs (miRNAs) can transfer biological information between cells and tissues and may act as potential biomarkers in many diseases. In this pilot study, we assessed the miRNA profile of exosomes released from human monocyte-derived macrophages upon infection with Mycobacterium bovis Bacillus Calmette-Guerin (BCG).
methodsHuman monocytes were obtained from the peripheral blood of three healthy subjects and driven to a monocyte-derived macrophage (MDM) phenotype using standard protocols. MDMs were infected with BCG or left uninfected as control. 72 h post-infection, exosomes were collected from the cell culture medium, RNA was isolated and RNA-seq performed. The raw reads were filtered to eliminate adaptor and primer sequences and the sequences were run against the mature human miRNA sequences available in miRBase. MicroRNAs were identified using an E value <0.01. miRNA network analysis was performed using the DIANA miRNA tool, miRDB and functional KEGG pathway analysis.
resultsInfection of MDMs with BCG leads to the release of several exosomal miRNAs. These included miR-1224, -1293, -425, -4467, -4732, -484, -5094, -6848-6849, -4488 and -96 all of which were predicted to target metabolism and energy production-related pathways.
conclusionsThis study provides evidence for the release of specific exosomal miRNAs from BCG-infected MDMs. These exosomal miRNAs reflect host-pathogen interaction and subversion of host metabolic processes following infection.
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