Evidence map›Paper›PMID 28499434›Full record

ArticleBMC veterinary research2017

Development of a novel immunochromatographic lateral flow assay specific for Mycobacterium bovis cells and its application in combination with immunomagnetic separation to test badger faeces.

Linda D Stewart, Nuria Tort, Paul Meakin, Jose M Argudo, Ruramayi Nzuma, Neil Reid, Richard J Delahay, Roland Ashford, W Ian Montgomery, Irene R Grant

Open access · goldAbstract readEvaluation Study
In one paragraph

Article in BMC veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
0.7field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 27 citations in OpenAlex.

  1. Pathogens (Basel, Switzerland) · 2025
    Review
  2. Article
  3. Review
  4. Article
  5. Review
  6. Article
  7. Article
  8. Metabotyping the Welsh population of badgers based on thoracic fluid.Metabolomics : Official journal of the Metabolomic Society · 2022
    Article
  9. Rapid detection ofRSC advances · 2021
    Article
  10. Review
  11. DoesVeterinary medicine international · 2021
    Review
  12. Validation of laboratory tests for infectious diseases in wild mammals: review and recommendations.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020
    Review
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Linda D StewartInstitute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, Northern Ireland, UK.
Nuria TortForsite Diagnostics Limited (now Abingdon Health), National Innovation Campus, Sand Hutton, York, UK.
Paul MeakinForsite Diagnostics Limited (now Abingdon Health), National Innovation Campus, Sand Hutton, York, UK.
Jose M ArgudoInstitute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, Northern Ireland, UK.
Ruramayi NzumaInstitute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, Northern Ireland, UK.
Neil ReidInstitute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, Northern Ireland, UK.
Richard J DelahayNational Wildlife Management Centre, Animal and Plant Health Agency, Woodchester Park, Nympsfield, Gloucestershire, UK.
Roland AshfordAnimal and Plant Health Agency, Weybridge, New Haw, Addlestone, Surrey, UK.
W Ian MontgomeryInstitute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, Northern Ireland, UK.
Irene R GrantInstitute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, Northern Ireland, UK. i.grant@qub.ac.uk.ORCID http://orcid.org/0000-0001-5860-3769
Queen's University Belfast · GBAbingdon Health (United Kingdom) · GBAnimal and Plant Health Agency · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe European badger is an important wildlife reservoir of Mycobacterium bovis implicated in the spread of bovine tuberculosis in the United Kingdom and Ireland. Infected badgers are known to shed M. bovis in their urine and faeces, which may contaminate the environment. To aid bovine tuberculosis control efforts novel diagnostic tests for detecting infected and shedding badgers are needed. We proposed development of a novel, rapid immunochromatographic lateral flow device (LFD) as a non-invasive test to detect M. bovis cells in badger faeces. Its application in combination with immunomagnetic separation (IMS) to detect Mycobacterium bovis cells in badger faeces is reported here.

resultsA novel prototype LFD for M. bovis cells was successfully developed, with unique specificity for M. bovis and a limit of detection 50% (LOD

conclusionsThe novel IMS-LFD assay, although very specific for M. bovis, has low analytical sensitivity (indicated by the LOD

Indexed as

AnimalsAntibodies, BacterialChromatography, AffinityFecesImmunomagnetic SeparationMustelidaeMycobacterium bovisSensitivity and SpecificityAntibodies, BacterialDiagnostic sensitivityDiagnostic specificityEuropean badger (Meles meles)Faecesimmunomagnetic separationLateral flow deviceMycobacterium bovisNon-invasive test

Identifiers

PMID28499434
PMCPMC5427577
OpenAlexW2612611132

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.