ArticleThe ISME journal2017
Genomic exploration of individual giant ocean viruses.
Article in The ISME journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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Who cites it
33 citing papers in PubMed, 50 citations in OpenAlex.
- The biogeography of giant viruses and interactions with Eukaryotes across European lakes.Nature communications · 2026Article
- Reducing haystacks to needles-ViralClust: a Nextflow pipeline to cluster viral sequences.GigaScience · 2026Article
- Single-particle genomics uncovers abundant non-canonical marine viruses from nanolitre volumes.Nature microbiology · 2025Article
- Identification and profiling of novel metagenome assembled uncultivated virus genomes from human gut.Virology journal · 2025Article
- Article
- Single-cell RNA-seq of the rare virosphere reveals the native hosts of giant viruses in the marine environment.Nature microbiology · 2024Article
- New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves.mSphere · 2024Article
- Virologs, viral mimicry, and virocell metabolism: the expanding scale of cellular functions encoded in the complex genomes of giant viruses.FEMS microbiology reviews · 2023Article
- Homing in on the rare virosphere reveals the native host of giant viruses.bioRxiv : the preprint server for biology · 2023Article
- Giant virus biology and diversity in the era of genome-resolved metagenomics.Nature reviews. Microbiology · 2022Review
- Antigen Presentation and Autophagy in Teleost Adaptive Immunity.International journal of molecular sciences · 2022Review
- Article
- Exploring Viral Diversity in a Gypsum Karst Lake Ecosystem Using Targeted Single-Cell Genomics.Genes · 2021Article
- Shrinking of repeating unit length in leucine-rich repeats from double-stranded DNA viruses.Archives of virology · 2021Article
- Metatranscriptome Library Preparation Influences Analyses of Viral Community Activity During a Brown Tide Bloom.Frontiers in microbiology · 2021Article
- Morphological and Genomic Features of the New Klosneuvirinae Isolate Fadolivirus IHUMI-VV54.Frontiers in microbiology · 2021Article
- Review
- Article
- Targeted metagenomic recovery of four divergent viruses reveals shared and distinctive characteristics of giant viruses of marine eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Parasite microbiome project: Grand challenges.PLoS pathogens · 2019Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viruses are major pathogens in all biological systems. Virus propagation and downstream analysis remains a challenge, particularly in the ocean where the majority of their microbial hosts remain recalcitrant to current culturing techniques. We used a cultivation-independent approach to isolate and sequence individual viruses. The protocol uses high-speed fluorescence-activated virus sorting flow cytometry, multiple displacement amplification (MDA), and downstream genomic sequencing. We focused on 'giant viruses' that are readily distinguishable by flow cytometry. From a single-milliliter sample of seawater collected from off the dock at Boothbay Harbor, ME, USA, we sorted almost 700 single virus particles, and subsequently focused on a detailed genome analysis of 12. A wide diversity of viruses was identified that included Iridoviridae, extended Mimiviridae and even a taxonomically novel (unresolved) giant virus. We discovered a viral metacaspase homolog in one of our sorted virus particles and discussed its implications in rewiring host metabolism to enhance infection. In addition, we demonstrated that viral metacaspases are widespread in the ocean. We also discovered a virus that contains both a reverse transcriptase and a transposase; although highly speculative, we suggest such a genetic complement would potentially allow this virus to exploit a latency propagation mechanism. Application of single virus genomics provides a powerful opportunity to circumvent cultivation of viruses, moving directly to genomic investigation of naturally occurring viruses, with the assurance that the sequence data is virus-specific, non-chimeric and contains no cellular contamination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.