ArticleJournal of nephrology2017
Decreased miR-128 and increased miR-21 synergistically cause podocyte injury in sepsis.
Article in Journal of nephrology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 20 citations in OpenAlex.
- Identification of candidate microRNA biomarkers in diabetic nephropathy: a meta-analysis of profiling studies.Journal of nephrology · 2018Pooled it
- MicroRNA Profiles in Critically Ill Patients.Current medicinal chemistry · 2024Review
- Preventive effect of hyperforin on lipopolysaccharide-induced acute kidney injury and inflammation by repressing the NF-κB/miR-21 axis.Central-European journal of immunology · 2024Article
- Naringin improves sepsis-induced intestinal injury by modulating macrophage polarization via PPARγ/miR-21 axis.Molecular therapy. Nucleic acids · 2021Article
- Serum and Glucocorticoid-Inducible Kinase 3/Nedd4-2 Signaling Pathway Participates in Podocyte Injury by Regulating the Stability of Nephrin.Frontiers in physiology · 2021Article
- miR-128-3p inhibits apoptosis and inflammation in LPS-induced sepsis by targeting TGFBR2.Open medicine (Warsaw, Poland) · 2021Article
- miR-21-5p inhibits inflammation injuries in LPS-treated H9c2 cells by regulating PDCD4.American journal of translational research · 2021Article
- Potential role of lncRNA HULC/miR‑128‑3p/RAC1 axis in the inflammatory response during LPS‑induced sepsis in HMEC‑1 cells.Molecular medicine reports · 2020Article
- MicroRNA-21-5p participates in IgA nephropathy by driving T helper cell polarization.Journal of nephrology · 2020Article
- Aberrant expression of miR-21 in patients with inflammatory bowel disease: A protocol for systematic review and meta analysis.Medicine · 2020Article
- Screening key genes and microRNAs in sepsis by RNA-sequencing.Journal of the Chinese Medical Association : JCMA · 2020Article
- MiR-106a aggravates sepsis-induced acute kidney injury by targeting THBS2 in mice model.Acta cirurgica brasileira · 2019Article
- Delayed Remote Ischemic Preconditioning ConfersRenoprotection against Septic Acute Kidney Injury via Exosomal miR-21.Theranostics · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveGlomerular podocytes are injured in sepsis. We studied, in a sepsis patient, whether microRNAs (miRNAs) play a role in the podocyte injury.
methodsPodocytes were cultured and treated with lipopolysaccharide (LPS). Filtration barrier function of podocyte was analyzed with albumin influx assay. Nephrin level was analyzed with reverse transcription polymerase chain reaction (RT-PCR) and western blot. MiRNAs were detected using miRNAs PCR Array and in situ hybridization. MiRNA target sites were evaluated with luciferase reporter assays.
resultsLPS impaired the filtration barrier function of podocytes. MiR-128 level was decreased and miR-21 level was increased in podocytes in vitro and in the sepsis patient. The decrease in miR-128 was sufficient to induce the loss of nephrin and the impairment of filtration barrier function, while the increase of miR-21 exacerbated the process. Snail and phosphatase and tensin homolog (PTEN) were identified as the targets of miR-128 and miR-21. Decreased miR-128 induced Snail expression, and the increased miR-21 stabilized Snail by regulating the PTEN/Akt/GSK3β pathway. Supplementation of miR-128 and inhibition of miR-21 suppressed Snail expression and prevented the podocyte injury induced by LPS.
conclusionOur study suggests that decreased miR-128 and increased miR-21 synergistically cause podocyte injury and are the potential therapeutic targets in sepsis.
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