Evidence map›Paper›PMID 28497335›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells.

Alena P Chumanevich, Piper A Wedman, Carole A Oskeritzian

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 93% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Alena P ChumanevichDepartment of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Building 2, Room C10, 6439 Garners Ferry Road, Columbia, SC, 20209, USA.
Piper A WedmanDepartment of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Building 2, Room C10, 6439 Garners Ferry Road, Columbia, SC, 20209, USA.
Carole A OskeritzianDepartment of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Building 2, Room C10, 6439 Garners Ferry Road, Columbia, SC, 20209, USA. Carole.Oskeritzian@uscmed.sc.edu.
University of South Carolina · US

Funding

Mast Cell S1P Receptor2 in the initiation and progression of chronic inflammationR01AI095494 · NIAID · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI OSKERITZIAN, CAROLE A · 2012 to 2015
$1.4M
MicroRNAs in atopic dermatitis pathogenesis: a role for mast cell and sphingosine-1-phosphate?R21AR067996 · NIAMS · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI OSKERITZIAN, CAROLE A · 2016 to 2017
$323k
NIAID NIH HHS R01 AI095494NIAMS NIH HHS R21 AR067996
6 · The paper itself

Abstract

Mast cells produce a potently bioactive sphingolipid metabolite sphingosine-1-phosphate (S1P) constitutively and upon activation. The ligation of S1P to its type 2 receptor on mast cells triggers a novel downstream signaling pathway that we discovered links activation of transcription factor signal transducer and activator of transcription 3 to mast cell-derived chemokine release in both humans and mice. In this chapter, we describe the methods used to study S1P signaling in human and mouse primary mast cells.

Indexed as

Signal TransductionAnimalsCells, CulturedChemokinesHumansLysophospholipidsMast CellsMiceSphingosineTranscription FactorsChemokinesLysophospholipidsSphingosinesphingosine 1-phosphateTranscription FactorsActivationChemokinesInflammationPrimary mast cellsProtein phosphorylationQuantitative PCRSignalingSignal transducer and activator of transcription 3Sphingosine-1-phosphateWestern blot

Identifiers

PMID28497335
PMCPMC10127149
OpenAlexW2613535464

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.