ArticleScientific reports2017
Overexpression of a cytochrome P450 and a UDP-glycosyltransferase is associated with imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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50 citing papers in PubMed, 150 citations in OpenAlex.
- Assessing the potential for insecticide cross-resistance to a novel insecticide in the Colorado potato beetle (Leptinotarsa decemlineata) using phenotypic and transcript expression analysis.Pest management science · 2026Article
- Transcriptome Analysis and Characterization of Two Detoxification Genes Responding to Imidacloprid inInsects · 2026Article
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- Bulk Segregant Analysis Reveals Genomic Regions Associated With Imidacloprid Resistance in the Colorado Potato Beetle.Ecology and evolution · 2025Article
- Tetraniliprole Triggers Transgenerational Hormesis in an Invasive Insect Herbivore: Molecular and Biological Insights.Insects · 2025Article
- Over-expression and increased copy numbers of a cytochrome P450 and two UDP-glucuronosyltransferase genes in macrocyclic lactone resistant Psoroptes ovis of cattle.PLoS pathogens · 2025Article
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- CRISPR/Cas Technology in Insect Insecticide Resistance.Insects · 2025Review
- Transgenic overexpression of UDP glycosyltransferase gene UGT41A3 induces resistance to nucleopolyhedrovirus in Bombyx mori.Transgenic research · 2024Article
- Field-evolved resistance to neonicotinoids in the mosquito, Anopheles gambiae, is associated with mutations of nicotinic acetylcholine receptor subunits combined with cytochrome P450-mediated detoxification.Pesticide biochemistry and physiology · 2024Article
- Field-evolved resistance to neonicotinoids in the mosquito,bioRxiv : the preprint server for biology · 2024Article
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- The impact of agrochemical pollutant mixtures on the selection of insecticide resistance in the malaria vector Anopheles gambiae: insights from experimental evolution and transcriptomics.Malaria journal · 2024Article
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- Leveraging RNA Interference to Impact Insecticide Resistance in the Colorado Potato Beetle,Insects · 2023Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current control of insect pests relies on chemical insecticides, however, insecticide resistance development by pests is a growing concern in pest management. The main mechanisms for insecticide resistance typically involve elevated activity of detoxifying enzymes and xenobiotic transporters that break-down and excrete insecticide molecules. In this study, we investigated the molecular mechanisms of imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), an insect pest notorious for its capacity to develop insecticide resistance rapidly. We compared the transcriptome profiles of imidacloprid-resistant and sensitive beetle strains and identified 102 differentially expressed transcripts encoding detoxifying enzymes and xenobiotic transporters. Of these, 74 were up-regulated and 28 were down-regulated in the resistant strain. We then used RNA interference to knock down the transcript levels of seven up-regulated genes in the resistant beetles. Ingestion of double-stranded RNA successfully knocked down the expression of the genes for three cytochrome P450s (CYP6BQ15, CYP4Q3 and CYP4Q7), one ATP binding cassette (ABC) transporter (ABC-G), one esterase (EST1), and two UDP-glycosyltransferases (UGT1 and UGT2). Further, we demonstrated that silencing of CYP4Q3 and UGT2 significantly increased susceptibility of resistant beetles to imidacloprid, indicating that overexpression of these two genes contributes to imidacloprid resistance in this resistant strain.
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