ArticleScientific reports2017
Measurement of local temperature increments induced by cultured HepG2 cells with micro-thermocouples in a thermally stabilized system.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Chip Calorimetry for Single-Cell Analysis: Advances, Challenges, and Opportunities.Sensors (Basel, Switzerland) · 2026Review
- A novel microfluidic chip integrated with Pt micro-thermometer for temperature measurement at the single-cell level.Heliyon · 2024Article
- Warm Cells, Hot Mitochondria: Achievements and Problems of Ultralocal Thermometry.International journal of molecular sciences · 2023Review
- Hydrogel Micropillar Array for Temperature Sensing in Fluid.IEEE sensors journal · 2023Article
- Thermal Probing Techniques for a Single Live Cell.Sensors (Basel, Switzerland) · 2022Review
- Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells.Biosensors · 2021Article
- Numerical modelling and experimental verification of thermal effects in living cells exposed to high-power pulses of THz radiation.Scientific reports · 2021Article
- A Micron-Sized Laser Photothermal Effect Evaluation System and Method.Sensors (Basel, Switzerland) · 2021Article
- Nanodiamond Theranostic for Light-Controlled Intracellular Heating and Nanoscale Temperature Sensing.Nano letters · 2021Article
- Wide-field fluorescent nanodiamond spin measurements toward real-time large-area intracellular thermometry.Scientific reports · 2021Article
- Thermophoretic Micron-Scale Devices: Practical Approach and Review.Entropy (Basel, Switzerland) · 2020Review
- Single-cell temperature mapping with fluorescent thermometer nanosheets.The Journal of general physiology · 2020Article
- The challenge of intracellular temperature.Biophysical reviews · 2020Review
- Optical Detection of Intracellular Quantities Using Nanoscale Technologies.Accounts of chemical research · 2019Article
- Multifunctional Freestanding Microprobes for Potential Biological Applications.Sensors (Basel, Switzerland) · 2019Article
- GFP fluorescence peak fraction analysis based nanothermometer for the assessment of exothermal mitochondria activity in live cells.Scientific reports · 2019Article
- Transient heat release during induced mitochondrial proton uncoupling.Communications biology · 2019Article
- Article
- Thermal sensing in fluid at the micro-nano-scales.Biomicrofluidics · 2018Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To monitor the temperature distribution of a cell and its changes under varied conditions is currently a technical challenge. A variety of non-contact methods used for measuring cellular temperature have been developed, where changes of local temperature at cell-level and sub-cell-level are indirectly calculated through the changes in intensity, band-shape, bandwidth, lifetime or polarization anisotropy of the fluorescence spectra recorded from the nano-sized fluorescent materials pre-injected into the target cell. Unfortunately, the optical properties of the fluorescent nano-materials may be affected by complicated intracellular environment, leading to unexpected measurement errors and controversial arguments. Here, we attempted to offer an alternative approach for measuring the absolute increments of local temperature in micro-Testing Zones induced by live cells. In this method, built-in high-performance micro-thermocouple arrays and double-stabilized system with a stability of 10 mK were applied. Increments of local temperature close to adherent human hepatoblastoma (HepG2) cells were continuously recorded for days without stimulus, showing frequent fluctuations within 60 mK and a maximum increment by 285 mK. This method may open a door for real-time recording of the absolute local temperature increments of individual cells, therefore offering valuable information for cell biology and clinical therapy in the field of cancer research.
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