ArticleBiophysical journal2017
Molecular Counting with Localization Microscopy: A Bayesian Estimate Based on Fluorophore Statistics.
Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- A Bayesian Model to Count the Number of Two-State Emitters in a Diffraction Limited Spot.Nano letters · 2025Article
- Single-molecule counting applied to the study of GPCR oligomerization.Biophysical journal · 2022Review
- Calibration-free counting of low molecular copy numbers in single DNA-PAINT localization clusters.Biophysical reports · 2021Article
- Estimating the dynamic range of quantitative single-molecule localization microscopy.Biophysical journal · 2021Article
- A pairwise distance distribution correction (DDC) algorithm to eliminate blinking-caused artifacts in SMLM.Nature methods · 2021Article
- An expectation-maximization approach to quantifying protein stoichiometry with single-molecule imaging.Bioinformatics advances · 2021Article
- Microampere Electric Current Causes Bacterial Membrane Damage and Two-Way Leakage in a Short Period of Time.Applied and environmental microbiology · 2020Article
- The Decade of Super-Resolution Microscopy of the Presynapse.Frontiers in synaptic neuroscience · 2020Review
- Toward Absolute Molecular Numbers in DNA-PAINT.Nano letters · 2019Article
- Single-molecule localization microscopy as nonlinear inverse problem.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- The Effect of Fluorophore Conjugation on Antibody Affinity and the Photophysical Properties of Dyes.Biophysical journal · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Superresolved localization microscopy has the potential to serve as an accurate, single-cell technique for counting the abundance of intracellular molecules. However, the stochastic blinking of single fluorophores can introduce large uncertainties into the final count. Here we provide a theoretical foundation for applying superresolved localization microscopy to the problem of molecular counting based on the distribution of blinking events from a single fluorophore. We also show that by redundantly tagging single molecules with multiple, blinking fluorophores, the accuracy of the technique can be enhanced by harnessing the central limit theorem. The coefficient of variation then, for the number of molecules M estimated from a given number of blinks B, scales like ∼1/N
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.