Evidence map›Paper›PMID 28494852›Full record

ArticleJournal of integrative medicine2017

Atomic force microscopy correlates antimetastatic potentials of HepG2 cell line with its redox/energy status: effects of curcumin and Khaya senegalensis.

Jeremiah Olorunjuwon Olugbami, Robert Damoiseaux, Bryan France, Michael A Gbadegesin, Adam Z Stieg, Shivani Sharma, Oyeronke A Odunola, James K Gimzewski

Abstract read
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In one paragraph

Article in Journal of integrative medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 18 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 3 countries.

Jeremiah Olorunjuwon OlugbamiCancer Research and Molecular Biology Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Oyo 200005, Nigeria.
Robert DamoiseauxMolecular Screening and Shared Resources Laboratories, California NanoSystems Institute, University of California, Los Angeles, California 90095, USA.
Bryan FranceMolecular Screening and Shared Resources Laboratories, California NanoSystems Institute, University of California, Los Angeles, California 90095, USA.
Michael A GbadegesinCancer Research and Molecular Biology Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Oyo 200005, Nigeria.
Adam Z StiegNano and Pico Characterization Laboratories, California NanoSystems Institute, University of California, Los Angeles, California 90095, USA.
Shivani SharmaNano and Pico Characterization Laboratories, California NanoSystems Institute, University of California, Los Angeles, California 90095, USA.
Oyeronke A OdunolaCancer Research and Molecular Biology Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Oyo 200005, Nigeria.
James K GimzewskiNano and Pico Characterization Laboratories, California NanoSystems Institute, University of California, Los Angeles, California 90095, USA.
California NanoSystems Institute · USUniversity of California, Los Angeles · USUniversity of Ibadan · NG

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Samuel Wheeler French · 1985 to 2026
$134.5M
NCI NIH HHS P30 CA016042
6 · The paper itself

Abstract

objectiveThe fatality of cancer is mostly dependent on the possibility of occurrence of metastasis. Thus, if the development of metastasis can be prevented through novel therapeutic strategies targeted against this process, then the success of cancer treatment will drastically increase. In this study, therefore, we evaluated the antimetastatic potentials of an extract of Khaya senegalensis and curcumin on the metastatic liver cell line HepG2, and also assessed the anticancer property of the extract.

methodsCells were cultured and treated with graded concentrations of test substances for 24, 48, or 72 h with provisions made for negative controls. Treated cells were assessed as follows: nanotechnologically - atomic force microscopy (AFM) was used to determine cell stiffness; biochemically - cell cytotoxicity, glutathione level and adenosine triphosphate status, caspase activation and mitochondrial toxicity were considered; and microbiologically - a carrot disk assay was used to assess the anticancer property of the extract of K. senegalensis.

resultsCurcumin and K. senegalensis increased the cell stiffness by 2.6- and 4.0-fold respectively, indicating their antimetastatic effects. Corresponding changes in redox (glutathione level) and energy (adenosine triphosphate) status of the cells were also demonstrated. Further mechanistic studies indicated that curcumin was not mitotoxic in HepG2 cells unlike the K. senegalensis extract. In addition, the extract potently inhibited the Agrobacterium tumefaciens-induced genetic transformation based on carrot disk assay.

conclusionCell elasticity measurement data, using AFM, strongly suggested, for the first time, that both curcumin and the extract of K. senegalensis exhibited antimetastatic properties on HepG2 cells.

Indexed as

CurcumaMeliaceaePhytotherapyAdenosine TriphosphateAntineoplastic Agents, PhytogenicApoptosisCell ProliferationCurcuminElasticityGlutathioneHep G2 CellsHumansMicroscopy, Atomic ForceNeoplasm InvasivenessNeoplasm MetastasisOxidation-ReductionAdenosine TriphosphateAntineoplastic Agents, PhytogenicCurcuminGlutathionePlant Extracts

Identifiers

PMID28494852
PMCPMC6497468
OpenAlexW2616776450

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.