ArticleBiomedical engineering online2017
Preparation and characterization of small-diameter decellularized scaffolds for vascular tissue engineering in an animal model.
Article in Biomedical engineering online, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Tissue compliance characterization for human umbilical arteries after decellularization.Journal of the mechanical behavior of biomedical materials · 2026Article
- Decellularized Nerve Scaffold in a Rat Model of Extensive Peripheral Nerve Damage.Sovremennye tekhnologii v meditsine · 2025Article
- The influence of physical and spatial substrate characteristics on endothelial cells.Materials today. Bio · 2024Review
- The journey of decellularized vessel: from laboratory to operating room.Frontiers in bioengineering and biotechnology · 2024Review
- From waste to wealth: Repurposing slaughterhouse waste for xenotransplantation.Frontiers in bioengineering and biotechnology · 2023Article
- Preliminary study of improving immune tolerance in vivo of bioprosthetic heart valves through a novel antigenic removal method.Frontiers in bioengineering and biotechnology · 2023Article
- Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering.Journal of functional biomaterials · 2022Review
- Monocyte Recruitment for Vascular Tissue Regeneration.Advanced healthcare materials · 2022Article
- Small-diameter artery decellularization: Effects of anionic detergent concentration and treatment duration on porcine internal thoracic arteries.Journal of biomedical materials research. Part B, Applied biomaterials · 2022Article
- Decellularized In Vitro Capillaries for Studies of Metastatic Tendency and Selection of Treatment.Biomedicines · 2022Article
- The hybrid crosslinking method improved the stability and anti-calcification properties of the bioprosthetic heart valves.Frontiers in bioengineering and biotechnology · 2022Article
- Photooxidation and Pentagalloyl Glucose Cross-Linking Improves the Performance of Decellularized Small-Diameter Vascular XenograftFrontiers in bioengineering and biotechnology · 2022Article
- Improved Cytocompatibility and Reduced Calcification of Glutaraldehyde-Crosslinked Bovine Pericardium by Modification With Glutathione.Frontiers in bioengineering and biotechnology · 2022Article
- Decellularized blood vessel development: Current state-of-the-art and future directions.Frontiers in bioengineering and biotechnology · 2022Review
- Yak Pericardium as an Alternative Biomaterial for Transcatheter Heart Valves.Frontiers in bioengineering and biotechnology · 2021Article
- Development of Acellular Respiratory Mucosal Matrix Using Porcine Tracheal Mucosa.Tissue engineering and regenerative medicine · 2020Article
- Endothelialization of arterial vascular grafts by circulating monocytes.Nature communications · 2020Article
- Sequential adaptation of perfusion and transport conditions significantly improves vascular construct recellularization and biomechanics.Journal of tissue engineering and regenerative medicine · 2020Article
- Implantation of VEGF-functionalized cell-free vascular grafts: regenerative and immunological response.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Building Blood Vessels with Vascular Progenitor Cells.Trends in molecular medicine · 2018Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe development of a suitable extracellular matrix (ECM) scaffold is the first step in vascular tissue engineering (VTE). Synthetic vascular grafts are available as an alternative to autologous vessels in large-diameter arteries (>8 mm) and medium-diameter arteries (6-8 mm). In small-diameter vessels (<6 mm), synthetic vascular grafts are of limited use due to poor patency rates. Compared with a vascular prosthesis, natural tissue ECM has valuable advantages. Despite considerable progress in recent years, identifying an optimal protocol to create a scaffold for use in small-diameter (<6 mm) fully natural tissue-engineered vascular grafts (TEVG), remains elusive. Although reports on different decellularization techniques have been numerous, combination of and comparison between these methods are scarce; therefore, we have compared five different decellularization protocols for making small-diameter (<6 mm) ECM scaffolds and evaluated their characteristics relative to those of fresh vascular controls.
resultsThe protocols differed in the choice of enzymatic digestion solvent, the use of non-ionic detergent, the durations of the individual steps, and UV crosslinking. Due to their small diameter and ready availability, rabbit arteria carotis were used as the source of the ECM scaffolds. The scaffolds were subcutaneously implanted in rats and the results were evaluated using various microscopy and immunostaining techniques.
conclusionsOur findings showed that a 2 h digestion time with 1× EDTA, replacing non-ionic detergent with double-distilled water for rinsing and the application of UV crosslinking gave rise to an ECM scaffold with the highest biocompatibility, lowest cytotoxicity and best mechanical properties for use in vivo or in situ pre-clinical research in VTE in comparison.
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