ArticlePloS one2017
No evidence of genome editing activity from Natronobacterium gregoryi Argonaute (NgAgo) in human cells.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Article
- Emerging Gene-editing nano-therapeutics for Cancer.Heliyon · 2024Review
- Targeting DNA polymerase to DNA double-strand breaks reduces DNA deletion size and increases templated insertions generated by CRISPR/Cas9.Nucleic acids research · 2022Article
- Identification of a Novel Cleavage Site and Confirmation of the Effectiveness of NgAgo Gene Editing on RNA Targets.Molecular biotechnology · 2021Article
- NgAgo possesses guided DNA nicking activity.Nucleic acids research · 2021Article
- Engineered extracellular vesicles as versatile ribonucleoprotein delivery vehicles for efficient and safe CRISPR genome editing.Journal of extracellular vesicles · 2021Article
- Does open data boost journal impact: evidence from Chinese economics.Scientometrics · 2021Article
- CRISPR/Cas9 increases mitotic gene conversion in human cells.Gene therapy · 2020Article
- Sensitive and reliable evaluation of single-cut sgRNAs to restore dystrophin by a GFP-reporter assay.PloS one · 2020Article
- Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient 'hit-and-run' genome editing.Nucleic acids research · 2019Article
- Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for transient expression and efficient genome editing.Nucleic acids research · 2019Article
- DNA interference and beyond: structure and functions of prokaryotic Argonaute proteins.Nature communications · 2018Review
- Fishing for understanding: Unlocking the zebrafish gene editor's toolbox.Methods (San Diego, Calif.) · 2018Review
- HPV Oncogene Manipulation Using Nonvirally Delivered CRISPR/Cas9 orAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Prokaryotic Argonaute proteins: novel genome-editing tools?Nature reviews. Microbiology · 2018Review
- Unexpected binding behaviors of bacterial Argonautes in human cells cast doubts on their use as targetable gene regulators.PloS one · 2018Article
- Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro.PloS one · 2018Article
- Beyond editing to writing large genomes.Nature reviews. Genetics · 2017Article
- Retraction: DNA-guided genome editing using the Natronobacterium gregoryi Argonaute.Nature biotechnology · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The argonaute protein from the thermophilic bacterium Thermus thermophilus shows DNA-guided DNA interfering activity at high temperatures, complicating its application in mammalian cells. A recent work reported that the argonaute protein from Natronobacterium gregoryi (NgAgo) had DNA-guided genome editing activity in mammalian cells. We compared the genome editing activities of NgAgo and Staphylococcus aureus Cas9 (SaCas9) in human HEK293T cells side by side. EGFP reporter assays and DNA sequencing consistently revealed high genome editing activity from SaCas9. However, these assays did not demonstrate genome editing activity by NgAgo. We confirmed that the conditions allowed simultaneous transfection of the NgAgo expressing plasmid DNA and DNA guides, as well as heterologous expression of NgAgo in the HEK293T cells. Our data show that NgAgo is not a robust genome editing tool, although it may have such activity under other conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.