ArticlePloS one2017
Pannexin 3 regulates proliferation and differentiation of odontoblasts via its hemichannel activities.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- Hydrostatic pressure promotes odontoblast differentiation via PIEZO1-dependent activation of RUNX2 and WNT16 in SHED.Scientific reports · 2026Article
- Pannexins in the musculoskeletal system: new targets for development and disease progression.Bone research · 2024Review
- Cryo-EM structures of pannexin 1 and 3 reveal differences among pannexin isoforms.Nature communications · 2024Article
- The functional effects of Piezo channels in mesenchymal stem cells.Stem cell research & therapy · 2023Review
- Pannexin1: insight into inflammatory conditions and its potential involvement in multiple organ dysfunction syndrome.Frontiers in immunology · 2023Review
- The odontoblastic differentiation of dental mesenchymal stem cells: molecular regulation mechanism and related genetic syndromes.Frontiers in cell and developmental biology · 2023Review
- Purinergic Signaling in Oral Tissues.International journal of molecular sciences · 2022Review
- KLF6 facilitates differentiation of odontoblasts through modulating the expression of P21 in vitro.International journal of oral science · 2022Article
- Mechanisms of Pannexin 1 (PANX1) Channel Mechanosensitivity and Its Pathological Roles.International journal of molecular sciences · 2022Review
- Expression of connexins and pannexins in diseased human liver.EXCLI journal · 2022Article
- Fibroblast growth factor 2 suppresses the expression of C-C motif chemokine 11 through the c-Jun N-terminal kinase pathway in human dental pulp-derived mesenchymal stem cells.Experimental and therapeutic medicine · 2021Article
- Pannexin 3 channels in health and disease.Purinergic signalling · 2021Review
- ATP transporters in the joints.Purinergic signalling · 2021Review
- Pannexin 3 regulates skin development via Epiprofin.Scientific reports · 2021Article
- Connexin 43-Mediated Gap Junction Communication Regulates Ameloblast DifferentiationFrontiers in physiology · 2021Article
- Unraveling the Role of the Apical Papilla During Dental Root Maturation.Frontiers in cell and developmental biology · 2021Review
- G protein-coupled receptorThe Journal of biological chemistry · 2020Article
- A Comparative In Vitro Analysis of the Osteogenic Potential of Human Dental Pulp Stem Cells Using Various Differentiation Conditions.International journal of molecular sciences · 2020Article
- Gelatinases Cleave Dentin Sialoprotein Intracellularly.Frontiers in physiology · 2020Article
- Regulation of miR-1-Mediated Connexin 43 Expression and Cell Proliferation in Dental Epithelial Cells.Frontiers in cell and developmental biology · 2020Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Highly coordinated regulation of cell proliferation and differentiation contributes to the formation of functionally shaped and sized teeth; however, the mechanism underlying the switch from cell cycle exit to cell differentiation during odontogenesis is poorly understood. Recently, we identified pannexin 3 (Panx3) as a member of the pannexin gap junction protein family from tooth germs. The expression of Panx3 was predominately localized in preodontoblasts that arise from dental papilla cells and can differentiate into dentin-secreting odontoblasts. Panx3 also co-localized with p21, a cyclin-dependent kinase inhibitor protein, in preodontoblasts. Panx3 was expressed in primary dental mesenchymal cells and in the mDP dental mesenchymal cell line. Both Panx3 and p21 were induced during the differentiation of mDP cells. Overexpression of Panx3 in mDP cells reduced cell proliferation via up-regulation of p21, but not of p27, and promoted the Bone morphogenetic protein 2 (BMP2)-induced phosphorylation of Smad1/5/8 and the expression of dentin sialophosphoprotein (Dspp), a marker of differentiated odontoblasts. Furthermore, Panx3 released intracellular ATP into the extracellular space through its hemichannel and induced the phosphorylation of AMP-activated protein kinase (AMPK). 5-Aminoimidazole-4-carboxamide-ribonucleoside (AICAR), an activator of AMPK, reduced mDP cell proliferation and induced p21 expression. Conversely, knockdown of endogenous Panx3 by siRNA inhibited AMPK phosphorylation, p21 expression, and the phosphorylation of Smad1/5/8 even in the presence of BMP2. Taken together, our results suggest that Panx3 modulates intracellular ATP levels, resulting in the inhibition of odontoblast proliferation through the AMPK/p21 signaling pathway and promotion of cell differentiation by the BMP/Smad signaling pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.