Evidence map›Paper›PMID 28493185›Full record

Trial reportLipids2017

Dietary PUFA Increase Apoptosis in Stomach of Patients with Dyspeptic Symptoms and Infected with H. pylori.

Rasoul Sharifi, Mohammad Nouri, Akram Eidi, Zahra Noormohammadi, Homayun Dolatkhah, Masoud Shirmohammadi

Abstract readClinical Trial
PubMed Publisher
In one paragraph

Trial report in Lipids, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 11 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Rasoul SharifiDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Mohammad NouriDepartment of Clinical Biochemistry and Laboratories Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, East-Azarbaijan, Iran. nourimd@yahoo.com.
Akram EidiDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Zahra NoormohammadiDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Homayun DolatkhahLiver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, East-Azarbaijan, Iran.
Masoud ShirmohammadiDepartment of Internal Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, East-Azarbaijan, Iran.
Islamic Azad University, Science and Research Branch · IRTabriz University of Medical Sciences · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Drug-resistant strains of Helicobacter pylori and poor treatment response are the main reasons for the failure in eradicating it in patients. Polyunsaturated fatty acids (PUFA) have an inhibitory effect on bacterial growth. The aim of this study was to investigate the effect of PUFA in combination with standard triple therapy on apoptosis in H. pylori infected subjects with dyspeptic symptoms. This study was a double-blind clinical trial in which 34 H. pylori infected subjects with dyspeptic symptoms were randomly divided into two groups of 17 patients. The control group received standard triple therapy (amoxicillin, clarithromycin and omeprazole) and the experimental group received the standard therapy and PUFA for two weeks. Gene expression levels of caspase-3, BCL-2 and Bad proteins were studied with real-time PCR, while protein levels were quantified in frozen sections and using immunohistochemistry. Compared with the control group, a significant increase (p < 0.01) was observed in the expression of caspase-3 and Bad genes and a significant reduction (p < 0.05) in the expression of Bcl-2 gene. The protein level of active caspase-3 and Bad protein was significantly increased and the level of Bcl-2 protein was significantly decreased (p < 0.05). The results of this study show that oral administration of PUFA in combination with the standard triple therapy increased apoptosis in H. pylori-infected patients with dyspeptic symptoms. This increase in apoptosis may partly reduce drug resistance in these patients. Our results suggest inclusion of a dietary PUFA containing fatty acid supplement may improve treatment of patients that are refractory to the standard triple therapy.

Indexed as

AdultAgedAmoxicillinAnti-Bacterial AgentsApoptosisCaspase 3ClarithromycinDietary Fats, UnsaturatedDouble-Blind MethodDyspepsiaFatty Acids, UnsaturatedFemaleGene Expression RegulationHelicobacter InfectionsHelicobacter pyloriHumansAmoxicillinAnti-Bacterial AgentsCaspase 3ClarithromycinDietary Fats, UnsaturatedFatty Acids, UnsaturatedOmeprazoleProto-Oncogene Proteins c-bcl-2ApoptosisDyspeptic symptomsHelicobacter pyloriUnsaturated fatty acids

Identifiers

PMID28493185
OpenAlexW2612454942

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.