ArticleJournal of the American Society for Mass Spectrometry2017
Overlapping MALDI-Mass Spectrometry Imaging for In-Parallel MS and MS/MS Data Acquisition without Sacrificing Spatial Resolution.
Article in Journal of the American Society for Mass Spectrometry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Mass Spectrometry Imaging.Analytical chemistry · 2025Review
- Enhancing Spatial Resolution in Tandem Mass Spectrometry Ion/Ion Reaction Imaging Experiments through Image Fusion.Journal of the American Society for Mass Spectrometry · 2024Article
- On-tissue dataset-dependent MALDI-TIMS-MSNature communications · 2023Article
- PICA: Pixel Intensity Correlation Analysis for Deconvolution and Metabolite Identification in Mass Spectrometry Imaging.Analytical chemistry · 2023Article
- Whole genome sequence-based characterization of virulence and antimicrobial resistance gene profiles ofFrontiers in microbiology · 2023Article
- Emerging Computational Methods in Mass Spectrometry Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Preparing ductal epithelial organoids for high-spatial-resolution molecular profiling using mass spectrometry imaging.Nature protocols · 2022Review
- In situ detection of fatty acid C=C positional isomers by coupling on-tissue mCPBA epoxidation with infrared matrix-assisted laser desorption electrospray ionization mass spectrometry.Rapid communications in mass spectrometry : RCM · 2021Article
- Automated annotation and visualisation of high-resolution spatial proteomic mass spectrometry imaging data using HIT-MAP.Nature communications · 2021Article
- Single-Cell Metabolomics by Mass Spectrometry Imaging.Advances in experimental medicine and biology · 2021Article
- High-Performance Molecular Imaging with MALDI Trapped Ion-Mobility Time-of-Flight (timsTOF) Mass Spectrometry.Analytical chemistry · 2019Article
- Towards resolving the spatial metabolome with unambiguous molecular annotations in complex biological systems by coupling mass spectrometry imaging with structures for lossless ion manipulations.Chemical communications (Cambridge, England) · 2019Article
- Imaging Isomers on a Biological Surface: A Review.Mass spectrometry (Tokyo, Japan) · 2019Article
- Balancing sufficiency and impact in reporting standards for mass spectrometry imaging experiments.GigaScience · 2018Article
- Automated, parallel mass spectrometry imaging and structural identification of lipids.Nature methods · 2018Article
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2 authors.
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Abstract
Metabolomics experiments require chemical identifications, often through MS/MS analysis. In mass spectrometry imaging (MSI), this necessitates running several serial tissue sections or using a multiplex data acquisition method. We have previously developed a multiplex MSI method to obtain MS and MS/MS data in a single experiment to acquire more chemical information in less data acquisition time. In this method, each raster step is composed of several spiral steps and each spiral step is used for a separate scan event (e.g., MS or MS/MS). One main limitation of this method is the loss of spatial resolution as the number of spiral steps increases, limiting its applicability for high-spatial resolution MSI. In this work, we demonstrate multiplex MS imaging is possible without sacrificing spatial resolution by the use of overlapping spiral steps, instead of spatially separated spiral steps as used in the previous work. Significant amounts of matrix and analytes are still left after multiple spectral acquisitions, especially with nanoparticle matrices, so that high quality MS and MS/MS data can be obtained on virtually the same tissue spot. This method was then applied to visualize metabolites and acquire their MS/MS spectra in maize leaf cross-sections at 10 μm spatial resolution. Graphical Abstract ᅟ.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.