ArticleScientific reports2017
DMBA acts on cumulus cells to desynchronize nuclear and cytoplasmic maturation of pig oocytes.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 32 citations in OpenAlex.
- Detection of c-Abl and mTERT expression by DMBA-ınduced mouse model of premature ovarian ınsufficiency.Journal of molecular histology · 2025Article
- Three-dimensional architecture of granulosa cell derived from oocyte cumulus complex, revealed by FIB-SEM.Journal of ovarian research · 2023Article
- Decreased oocyte quality in patients with endometriosis is closely related to abnormal granulosa cells.Frontiers in endocrinology · 2023Review
- Oocyte quality assessment in marine invertebrates: a novel approach by fluorescence spectroscopy.Biological research · 2022Article
- MicroRNA21 inhibition affects porcine oocyte maturation and alters protein expression critical for metabolic pathway function.Molecular reproduction and development · 2022Article
- SIRT2 Is Critical for Sheep Oocyte Maturation through Regulating Function of Surrounding Granulosa Cells.International journal of molecular sciences · 2022Article
- The effects of polycyclic aromatic compounds (PACs) on mammalian ovarian function.Current research in toxicology · 2022Article
- Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes.Genes · 2021Article
- An Efficient Nitroblue Tetrazolium Staining and Bright-Field Microscopy Based Method for Detecting and Quantifying Intracellular Reactive Oxygen Species in Oocytes, Cumulus Cells and Embryos.Frontiers in cell and developmental biology · 2020Article
- Autophagy is required for proper meiosis of porcine oocytes maturing in vitro.Scientific reports · 2018Article
- Heat shock protein 90α couples with the MAPK-signaling pathway to determine meiotic maturation of porcine oocytes.Journal of animal science · 2018Article
- Ascorbic acid induces global epigenetic reprogramming to promote meiotic maturation and developmental competence of porcine oocytes.Scientific reports · 2018Article
- Sodium fluoride exposure exerts toxic effects on porcine oocyte maturation.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As an environmental pollutant and carcinogen, 7,12-dimethylbenz[a]anthracene (DMBA) can destroy ovarian follicles at all developmental stages in rodents. However, the underlying molecular mechanism remains obscure. In the present study, we aim to address how DMBA affects the in vitro maturation and development of porcine oocytes. We discovered that for 20 μM DMBA-treated cumulus-oocyte complexes (COCs), the rate of oocyte germinal vesicle breakdown (GVBD) was significantly altered, and the extrusion rate of first polar body was increased. Moreover, oocytes from 20 μM DMBA-treated COCs had significant down-regulation of H3K9me3 and H3K27me3, up-regulation of H3K36me3, higher incidence of DNA double strand breaks (DSBs) and early apoptosis. In striking contrast, none of these changes happened to 20 μM DMBA-treated cumulus-denuded oocytes (CDOs). Furthermore, 20 μM DMBA treatment increased the reactive oxygen species (ROS) level, decreased mitochondrial membrane potential (Δ Ψm), and inhibited developmental competence for oocytes from both COC and CDO groups. Collectively, our data indicate DMBA could act on cumulus cells via the gap junction to disturb the synchronization of nuclear and ooplasmic maturation, and reduce the developmental competence of oocytes.
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