ArticleScientific reports2017
Nuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Identification of Hybrid Sturgeon (Animals : an open access journal from MDPI · 2025Article
- Comprehensive Assessment of Rapeseed Meal as a Fish Meal Substitute in Hybrid Sturgeon (Aquaculture nutrition · 2025Article
- The Effect of Cryopreserved Sperm on the Early Development, Survival, and Growth of Intergeneric Sterbel Hybrids (International journal of molecular sciences · 2024Article
- A Multistep DNA-Based Methodology for Accurate Authentication of Sturgeon Species.Foods (Basel, Switzerland) · 2022Article
- Intracytoplasmic sperm injection in sturgeon species: A promising reproductive technology of selected genitors.Frontiers in veterinary science · 2022Article
- Distant hybrids of Heliocidaris crassispina (♀) and Strongylocentrotus intermedius (♂): identification and mtDNA heteroplasmy analysis.BMC evolutionary biology · 2020Article
- A newly developed cloning technique in sturgeons; an important step towards recovering endangered species.Scientific reports · 2019Article
- Optimization of In Vitro Culture Conditions of Sturgeon Germ Cells for Purpose of Surrogate Production.Animals : an open access journal from MDPI · 2019Article
- Polyspermy produces viable haploid/diploid mosaics in sturgeon.Biology of reproduction · 2018Article
- Application of interspecific Somatic Cell Nuclear Transfer (iSCNT) in sturgeons and an unexpectedly produced gynogenetic sterlet with homozygous quadruple haploid.Scientific reports · 2018Article
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Sturgeons (Acipenseriformes) are among the most endangered species in the world due to fragmentation and destruction of their natural habitats and to overexploitation, mainly for highly priced caviar. This has led to the development of sturgeon culture, originally for reintroduction, but more recently for caviar production. In both cases, accurate species identification is essential. We report a new tool for accurate identification of Huso huso and Acipenser ruthenus based on nuclear DNA markers. We employed ddRAD sequencing to identify species-specific nucleotide variants, which served as specific binding sites for diagnostic primers. The primers allowed identification of Huso huso and Acipenser ruthenus as well as their discrimination from A. baerii, A. schrenckii, A. gueldenstaedtii, A. stellatus, A. persicus, A. mikadoi, A. transmontanus, and H. dauricus and identification of A. ruthenus and H. huso hybrids with these species, except hybrid between A. ruthenus and A. stellatus. The species-specific primers also allowed identification of bester (H. huso × A. ruthenus), the most commercially exploited sturgeon hybrid. The tool, based on simple PCR and gel electrophoresis, is rapid, inexpensive, and reproducible. It will contribute to conservation of remaining wild populations of A. ruthenus and H. huso, as well as to traceability of their products.
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