Multi-pass transmission electron microscopy.

Thomas Juffmann, Stewart A Koppell, Brannon B Klopfer, Colin Ophus, Robert M Glaeser, Mark A Kasevich

Abstract read

In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

Single-particle cryo-EM: alternative schemes to improve dose efficiency.Journal of synchrotron radiation · 2021
Article
An Algorithm for Enhancing the Image Contrast of Electron Tomography.Scientific reports · 2018
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Thomas JuffmannPhysics Department, Stanford University, 382 Via Pueblo Mall, Stanford, California, 94305, USA. juffmann@stanford.edu.ORCID 0000-0002-7098-5736

Stewart A KoppellPhysics Department, Stanford University, 382 Via Pueblo Mall, Stanford, California, 94305, USA.

Brannon B KlopferPhysics Department, Stanford University, 382 Via Pueblo Mall, Stanford, California, 94305, USA.

Colin OphusNational Center for Electron Microscopy, Molecular Foundry, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California, 94720, USA.

Robert M GlaeserMolecular Biophysics and Integrative Bioimaging, Lawrence Berkeley National Laboratory, University of California, Berkeley, California, 94720, USA.

Mark A KasevichPhysics Department, Stanford University, 382 Via Pueblo Mall, Stanford, California, 94305, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Feynman once asked physicists to build better electron microscopes to be able to watch biology at work. While electron microscopes can now provide atomic resolution, electron beam induced specimen damage precludes high resolution imaging of sensitive materials, such as single proteins or polymers. Here, we use simulations to show that an electron microscope based on a multi-pass measurement protocol enables imaging of single proteins, without averaging structures over multiple images. While we demonstrate the method for particular imaging targets, the approach is broadly applicable and is expected to improve resolution and sensitivity for a range of electron microscopy imaging modalities, including, for example, scanning and spectroscopic techniques. The approach implements a quantum mechanically optimal strategy which under idealized conditions can be considered interaction-free.

Identifiers

PMID28490730

DOI10.1038/s41598-017-01841-x

PMCPMC5431881

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.