ArticleRNA biology2017
Observing the fate of tRNA and its modifications by nucleic acid isotope labeling mass spectrometry: NAIL-MS.
Article in RNA biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 61 citations in OpenAlex.
- The Rare RNA Methylations m²G, Cm, m⁵U and ms²i⁶A: Roles in Disease Pathogenesis and Emerging Therapeutic Implications.International journal of medical sciences · 2026Review
- Selective Chemical Manipulation of Mannosyl- and Galactosyl-Queuosine in tRNAs of Living Cells.Angewandte Chemie (International ed. in English) · 2025Article
- Quantification of parathyroid hormone in high-purity materials by two isotope dilution mass spectrometry methods.Analytical and bioanalytical chemistry · 2025Article
- Detection, molecular function and mechanisms of m5C in cancer.Clinical and translational medicine · 2025Review
- Establishment of genomic RNA reference materials for BCR-ABL1 P210 measurement.Analytical and bioanalytical chemistry · 2024Article
- Complete list of canonical post-transcriptional modifications in the Bacillus subtilis ribosome and their link to RbgA driven large subunit assembly.Nucleic acids research · 2024Article
- Complete list of canonical post-transcriptional modifications in thebioRxiv : the preprint server for biology · 2024Article
- Temporal resolution of NAIL-MS of tRNA, rRNA and Poly-A RNA is overcome by actinomycin D.RSC chemical biology · 2023Article
- Expanding the Epitranscriptomic RNA Sequencing and Modification Mapping Mass Spectrometry Toolbox with Field Asymmetric Waveform Ion Mobility and Electrochemical Elution Liquid Chromatography.Analytical chemistry · 2023Article
- Opportunities and Challenges to Profile mRNA Modifications in Escherichia coli.Chembiochem : a European journal of chemical biology · 2022Article
- Quantification of mRNA cap-modifications by means of LC-QqQ-MS.Methods (San Diego, Calif.) · 2022Article
- Development of a human insulin certified reference material with SI-traceable purity.Analytical and bioanalytical chemistry · 2022Article
- Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry.Journal of visualized experiments : JoVE · 2022Article
- Advances in mRNA 5-methylcytosine modifications: Detection, effectors, biological functions, and clinical relevance.Molecular therapy. Nucleic acids · 2021Review
- The occurrence order and cross-talk of different tRNA modifications.Science China. Life sciences · 2021Review
- The Stress-Dependent Dynamics ofGenes · 2021Article
- The expanding world of tRNA modifications and their disease relevance.Nature reviews. Molecular cell biology · 2021Review
- Aging through an epitranscriptomic lens.Nature aging · 2021Review
- Cell culture NAIL-MS allows insight into human tRNA and rRNA modification dynamics in vivo.Nature communications · 2021Article
- Identification and rescue of a tRNA wobble inosine deficiency causing intellectual disability disorder.RNA (New York, N.Y.) · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA in yeast, especially rRNA and tRNA are heavily modified to fulfill their function in protein translation. Using biosynthetic stable isotope labeled internal standards we quantified 12 modified nucleosides in tRNA from S. cerevisiae over 24 hours. We observed different quantities of modified nucleosides in dependence of the growth phase. To elucidate the underlying mechanism of the observed tRNA modification profile adaptation, it is necessary to distinguish the pre-existing tRNA pool and its modifications from newly-synthesized tRNAs. By combination of 2 differentially isotope labeled media we developed NAIL-MS, nucleic acid isotope labeling coupled mass spectrometry. During the yeast growth cycle we observe dilution of pre-existing tRNAs by newly-synthesized tRNAs by the growing number of cells. tRNA was found to be highly stable with only little degradation over the observed period. The method was further used to quantify the levels of modified nucleosides in the original and new tRNA pools. By addition of deuterium-labeled methionine, we could observe the incorporation of new methyl marks on pre-existing tRNAs. For 2'-O-methylcytidine (Cm) we observed a global increase in log phase. We identified extensive 2'-OH-cytidine methylation of the pre-existing tRNAs and the new tRNAs which masks an actual decrease of pre-existing Cm. In contrast, global 5-methylcytidine (m
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.