Evidence map›Paper›PMID 28487957›Full record

ArticleMolecular medicine reports2017

Chemokine (C‑C motif) ligand 21/C‑C chemokine receptor type 7 triggers migration and invasion of human lung cancer cells by epithelial‑mesenchymal transition via the extracellular signal‑regulated kinase signaling pathway.

Guangxin Zhong, Lu Chen, Ruihong Yin, Yan Qu, Yongxing Bao, Qiong Xiao, Zhaolin Zhang, Yaqian Shen, Cailing Li, Yun Xu and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed, 1 pooled it
1.5field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Guangxin ZhongInstitute of Anatomy and Histology and Embryology, School of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China.
Lu ChenInstitute of Anatomy and Histology and Embryology, School of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China.
Ruihong YinDepartment of Internal Medicine, Jinan First People's Hospital, Jinan, Shandong 250000, P.R. China.
Yan QuInstitute of Anatomy and Histology and Embryology, School of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China.
Yongxing BaoInstitute of Anatomy and Histology and Embryology, School of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China.
Qiong XiaoBlood Center of General Hospital of Jinan Military Region, Jinan, Shandong 250031, P.R. China.
Zhaolin ZhangDepartment of Special Examination, Penglai People's Hospital, Penglai, Shandong 265600, P.R. China.
Yaqian ShenInstitute of Anatomy and Histology and Embryology, School of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China.
Cailing LiInstitute of Anatomy and Histology and Embryology, School of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China.
Yun XuDepartment of Anatomy, Jining Medical University, Jining, Shandong 272000, P.R. China.
Zhigeng ZouCancer Treatment Center, Shandong Provincial Hospital, Shandong University, Jinan, Shandong 250021, P.R. China.
Hua TianInstitute of Anatomy and Histology and Embryology, School of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China.
Shandong University · CNJinan City People's Hospital · CNJinan Military General Hospital · CNJining Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

C-C chemokine receptor type 7 (CCR7) has been implicated in lymph node metastasis of various cancers. Previous studies have revealed that epithelial‑mesenchymal transition (EMT) is involved in the chemotactic process mediated by CCR7 and its ligands in various types of carcinoma. However, the underlying mechanism of this process remains to be fully elucidated. The present study investigated whether chemokine (C‑C motif) ligand 21 (CCL21)/CCR7 may activate EMT of lung cancer cells and their associated signaling pathways. A549 and H520 lung cancer cell lines were examined in vitro in the present study. The results indicated that A549 and H520 expressed CCR7, but reduced levels of CCL21. Following stimulation of lung cancer cell lines with CCL21, the expression of the epithelial marker E‑cadherin was downregulated, and the mesenchymal markers Vimentin/Slug and extracellular signal‑regulated kinase (ERK) were upregulated. In addition, the ERK inhibitor PD98059 may inhibit EMT caused by CCL21, and decreased cell migration and invasion initiated by CCL21. Furthermore, lung adenocarcinoma tissues from 50 patients who underwent lung cancer operations were investigated by immunohistochemistry. The findings revealed that CCR7, Slug and Vimentin were highly expressed in lung carcinoma tissues, and were significantly associated with lymph node metastasis and clinical pathological stages, respectively. CCR7 expression was correlated positively with expression levels of Slug and Vimentin. CCL21 was expressed positively in the endothelium of lymphatic vessels adjacent to cancer cells, and weakly in lung cancer cells. Collectively, these results demonstrated that CCL21/CCR7 may activate EMT in lung cancer cells via the ERK1/2 signaling pathway. The current study provides evidence that a close interaction exists between CCL21/CCR7chemotaxis and EMT procedures in lung cancer metastasis, providing a basis for the development of therapeutic targets.

Indexed as

Epithelial-Mesenchymal TransitionSignal TransductionAdultAgedBiomarkersCell Line, TumorCell MovementChemokine CCL21Extracellular Signal-Regulated MAP KinasesFemaleGene ExpressionHumansLung NeoplasmsLymphatic MetastasisMaleMiddle AgedBiomarkersCCL21 protein, humanChemokine CCL21Extracellular Signal-Regulated MAP KinasesReceptors, CCR7SNAI1 protein, humanSnail Family Transcription FactorsVimentin

Identifiers

PMID28487957
PMCPMC5436267
OpenAlexW2611034210

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.