ArticlePloS one2017
Immortalization of chicken preadipocytes by retroviral transduction of chicken TERT and TR.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 94 citations in OpenAlex.
- Transcriptomic Analysis of High and Low Lipid Droplet Deposition Subpopulations of Chicken Preadipocytes Based on SSC Sorting.Animals : an open access journal from MDPI · 2026Article
- gga-let-7c-3p Inhibits Chicken Abdominal Adipogenesis by RegulatingBiomolecules · 2026Article
- Article
- PLIN1a: the principal protein isoform that performs the function of chicken PLIN1.Frontiers in cell and developmental biology · 2026Article
- Study on immortalization of Mongolian sheep fibroblast cells.Animal biotechnology · 2025Article
- Unravelling bovine preadipocyte differentiation and their three-dimensional cultivation for cellular agriculture.NPJ science of food · 2025Article
- KLF2 may suppress chicken adipogenesis by enhancing the GATA2 transcription.Scientific reports · 2025Article
- A unique spontaneously immortalised cell line from pig with enhanced adipogenic capacity.NPJ science of food · 2025Article
- The Effect ofAnimals : an open access journal from MDPI · 2025Article
- Integrative 3D genomics with multi-omics analysis and functional validation of genetic regulatory mechanisms of abdominal fat deposition in chickens.Nature communications · 2024Article
- Establishment and Characterization of a Chicken Myoblast Cell Line.International journal of molecular sciences · 2024Article
- ALKBH5 regulates chicken adipogenesis by mediating LCAT mRNA stability depending on mBMC genomics · 2024Article
- Article
- SSTR2 Mediates the Inhibitory Effect of SST/CST on Lipolysis in Chicken Adipose Tissue.Animals : an open access journal from MDPI · 2024Article
- Article
- Selection on the promoter regions plays an important role in complex traits during duck domestication.BMC biology · 2023Article
- An immortal porcine preadipocyte cell strain for efficient production of cell-cultured fat.Communications biology · 2023Article
- A Functional Variant Alters the Binding ofAnimals : an open access journal from MDPI · 2023Article
- Review
- A novel candidate gene CLN8 regulates fat deposition in avian.Journal of animal science and biotechnology · 2023Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chicken is an important agricultural animal and model for developmental biology, immunology and virology. Excess fat accumulation continues to be a serious problem for the chicken industry. However, chicken adipogenesis and obesity have not been well investigated, because no chicken preadipocyte cell lines have been generated thus far. Here, we successfully generated two immortalized chicken preadipocyte cell lines through transduction of either chicken telomerase reverse transcriptase (chTERT) alone or in combination with chicken telomerase RNA (chTR). Both of these cell lines have survived >100 population doublings in vitro, display high telomerase activity and have no sign of replicative senescence. Similar to primary chicken preadipocytes, these two cell lines display a fibroblast-like morphology, retain the capacity to differentiate into adipocytes, and do not display any signs of malignant transformation. Isoenzyme analysis and PCR-based analysis confirmed that these two cell lines are of chicken origin and are free from inter-species contamination. To our knowledge, this is the first report demonstrating the generation of immortal chicken cells by introduction of chTERT and chTR. Our established chicken preadipocyte cell lines show great promise as an in vitro model for the investigation of chicken adipogenesis, lipid metabolism, and obesity and its related diseases, and our results also provide clues for immortalizing other avian cell types.
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