ArticlePLoS computational biology2017
Single-molecule protein identification by sub-nanopore sensors.
Article in PLoS computational biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- The Potential of Nanopore Technologies in Peptide and Protein Sensing for Biomarker Detection.Biosensors · 2025Review
- Machine learning empowered next generation DNA sequencing: perspective and prospectus.Chemical science · 2024Review
- Whole protein sequencing and quantification without proteolysis, terminal residue cleavage, or purification: A computational model.bioRxiv : the preprint server for biology · 2024Article
- T-S2Inet: Transformer-based sequence-to-image network for accurate nanopore sequence recognition.Bioinformatics (Oxford, England) · 2024Article
- Review
- Active learning for efficient analysis of high-throughput nanopore data.Bioinformatics (Oxford, England) · 2023Article
- Calling the amino acid sequence of a protein/peptide from the nanospectrum produced by a sub-nanometer diameter pore.Scientific reports · 2022Article
- A Guide to Signal Processing Algorithms for Nanopore Sensors.ACS sensors · 2021Article
- Digital immunoassay for biomarker concentration quantification using solid-state nanopores.Nature communications · 2021Article
- Nanopore sensing: A physical-chemical approach.Biochimica et biophysica acta. Biomembranes · 2021Review
- Recent advances in integrated solid-state nanopore sensors.Lab on a chip · 2021Review
- A machine learning approach for accurate and real-time DNA sequence identification.BMC genomics · 2021Article
- Application of Solid-State Nanopore in Protein Detection.International journal of molecular sciences · 2020Review
- Strategies for Development of a Next-Generation Protein Sequencing Platform.Trends in biochemical sciences · 2020Review
- Beyond mass spectrometry, the next step in proteomics.Science advances · 2020Review
- Biophysics and the Genomic Sciences.Biophysical journal · 2019Review
- Protein fingerprinting with digital sequences of linear protein subsequence volumes: a computational study.Journal of biosciences · 2019Article
- FraC nanopores with adjustable diameter identify the mass of opposite-charge peptides with 44 dalton resolution.Nature communications · 2019Article
- Colloquium: Ionic phenomena in nanoscale pores through 2D materials.Reviews of modern physics · 2019Article
- A theoretical analysis of single molecule protein sequencing via weak binding spectra.PloS one · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Recent advances in top-down mass spectrometry enabled identification of intact proteins, but this technology still faces challenges. For example, top-down mass spectrometry suffers from a lack of sensitivity since the ion counts for a single fragmentation event are often low. In contrast, nanopore technology is exquisitely sensitive to single intact molecules, but it has only been successfully applied to DNA sequencing, so far. Here, we explore the potential of sub-nanopores for single-molecule protein identification (SMPI) and describe an algorithm for identification of the electrical current blockade signal (nanospectrum) resulting from the translocation of a denaturated, linearly charged protein through a sub-nanopore. The analysis of identification p-values suggests that the current technology is already sufficient for matching nanospectra against small protein databases, e.g., protein identification in bacterial proteomes.
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Registered trials
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