ArticleJournal of dental research2017
Arginine Improves pH Homeostasis via Metabolism and Microbiome Modulation.
Article in Journal of dental research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 81 citations in OpenAlex.
- Arginine modulates the pH, microbial composition, and matrix architecture of biofilms from caries-active patients.International journal of oral science · 2025Trial
- Effect of arginine-fluoride varnish on preventing enamel erosion by paediatric liquid medicaments.BMC oral health · 2023Trial
- Metabolic Profile of Supragingival Plaque Exposed to Arginine and Fluoride.Journal of dental research · 2019Trial
- [Age-Related Changes in the Oral Microbiota].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2026Review
- Iron, arginine, and redox metabolism in peripheral blood mononuclear cells distinguishes sickle cell disease and pulmonary hypertension.HemaSphere · 2026Article
- Aspartic acid-facilitated remineralization: a bio-inspired alternative to fluoride for enamel repair.Frontiers in bioengineering and biotechnology · 2026Article
- Oral hygiene agents at work: effects onFrontiers in cellular and infection microbiology · 2026Review
- Article
- Arginine-loaded mesoporous silica nanoparticles modified 3D-printed nanocomposite denture base resin with improved mechanical and antimicrobial properties.BMC oral health · 2025Article
- Nontargeted metabolomics analysis to unravel the anti-biofilm mechanism of Citrocin onMicrobiology spectrum · 2025Article
- Biofilm volume and acidification within initial biofilms formed in situ on buccally and palatally exposed bracket material.Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie · 2025Article
- Non-targeted metabolomic profile of Leuconostoc mesenteroides-fermented milk reveals differentially expressed metabolites associated with electro-fermentation.Microbial cell factories · 2025Article
- Synergistic effect of nanosilver fluoride with L-arginine on remineralization of early carious lesions.Scientific reports · 2025Article
- The effect of supplemental arginine on the gut microbial homeostasis of broilers during sub-clinical necrotic enteritis challenge.Frontiers in physiology · 2024Article
- The oral microbiome in the pathophysiology of cardiovascular disease.Nature reviews. Cardiology · 2023Review
- Antimicrobial Activity and Cytotoxicity of Nonsteroidal Anti-Inflammatory Drugs against Endodontic Biofilms.Antibiotics (Basel, Switzerland) · 2023Article
- Teg58, a small regulatory RNA, is involved in regulating arginine biosynthesis and biofilm formation in Staphylococcus aureus.Scientific reports · 2022Article
- The Arginine Biosynthesis Pathway of Candida albicans Regulates Its Cross-Kingdom Interaction with Actinomyces viscosus to Promote Root Caries.Microbiology spectrum · 2022Article
- Strategies to Combat Caries by Maintaining the Integrity of Biofilm and Homeostasis during the Rapid Phase of Supragingival Plaque Formation.Antibiotics (Basel, Switzerland) · 2022Review
- Nutrient Sensing and Biofilm Modulation: The Example of L-arginine inInternational journal of molecular sciences · 2022Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Dental caries can be described as a dysbiosis of the oral microbial community, in which acidogenic, aciduric, and acid-adapted bacterial species promote a pathogenic environment, leading to demineralization. Alkali generation by oral microbes, specifically via arginine catabolic pathways, is an essential factor in maintaining plaque pH homeostasis. There is evidence that the use of arginine in dentifrices helps protect against caries. The aim of the current study was to investigate the mechanistic and ecological effect of arginine treatment on the oral microbiome and its regulation of pH dynamics, using an in vitro multispecies oral biofilm model that was previously shown to be highly reflective of the in vivo oral microbiome. Pooled saliva from 6 healthy subjects was used to generate overnight biofilms, reflecting early stages of biofilm maturation. First, we investigated the uptake of arginine by the cells of the biofilm as well as the metabolites generated. We next explored the effect of arginine on pH dynamics by pretreating biofilms with 75 mM arginine, followed by the addition of sucrose (15 mM) after 0, 6, 20, or 48 h. pH was measured at each time point and biofilms were collected for 16S sequencing and targeted arginine quantification, and supernatants were prepared for metabolomic analysis. Treatment with only sucrose led to a sustained pH drop from 7 to 4.5, while biofilms treated with sucrose after 6, 20, or 48 h of preincubation with arginine exhibited a recovery to higher pH. Arginine was detected within the cells of the biofilms, indicating active uptake, and arginine catabolites citrulline, ornithine, and putrescine were detected in supernatants, indicating active metabolism. Sequencing analysis revealed a shift in the microbial community structure in arginine-treated biofilms as well as increased species diversity. Overall, we show that arginine improved pH homeostasis through a remodeling of the oral microbial community.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.